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Gene expression after low dose irradiation in pluripotent stem cells developing to retinal ganglion cells

ABSTRACT: Gene expression after low dose irradiation in pluripotent stem cells developing to retinal ganglion cells

PROVIDER: PRJNA597881 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10775462_1.fastq.gz	Fastqsanger.gz
	SRR10775462_2.fastq.gz	Fastqsanger.gz
	SRR10775463_1.fastq.gz	Fastqsanger.gz
	SRR10775463_2.fastq.gz	Fastqsanger.gz
	SRR10775464_1.fastq.gz	Fastqsanger.gz

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Gene expression after low dose irradiation in pluripotent stem cells developing to retinal ganglion cells

Project description:Gene expression after low dose irradiation in pluripotent stem cells developing to retinal ganglion cells

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Gene expression after low dose irradiation in pluripotent stem cells developing to retinal ganglion cells

Project description:The effects of low dose irradiation on the gene expression on the neural cell development using human induced pluripotent stem cells (hiPS cells).

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Gene expression after low dose irradiation in pluripotent stem cells developing to retinal ganglion cells

Project description:The effects of low dose irradiation on the gene expression on the neural cell development using human induced pluripotent stem cells (hiPS cells).

2020-12-26 | GSE142658 | GEO

Transcription profiling of chicken whole eye and retinal ganglion cells (RGC) to identify RGC-specific genes

Project description:Identification of genes regulated by ATH5 in a dose-dependent manner.; Identification of retinal ganglion cells (RGC)-specific genes.

2009-01-14 | E-TABM-417 | biostudies-arrayexpress

Transcription profiling of mouse developing retinal ganglion and amacrine cells reveals molecular heterogeneity of

Project description:During development of the central nervous system (CNS), cycling uncommitted progenitor cells give rise to a variety of distinct neuronal and glial cell types. As these different cell types are born, they progress from newly specified cells to fully differentiated neurons and glia. In order to define the developmental processes of individual cell types, single cell expression profiling was carried out on developing ganglion and amacrine cells of the murine retina. Individual cells from multiple developmental stages were isolated and profiled on Affymetrix oligonucleotide arrays. These experiments have yielded an expanded view of the processes underway in developing retinal ganglion and amacrine cells, as well as several hundred new marker genes for these cell types. In addition, this study has allowed for the definition of some of the molecular heterogeneity both between developing ganglion and amacrine cells and among subclasses of each cell type. Experiment Overall Design: Single retinal cells were isolated in tubes containing lysis buffer, their mRNAs were reverse transcribed, and the resulting cDNAs were PCR amplified for 35 cycles. Labeled cDNA samples were hybridized to Affymetrix 430 2.0 microarrays and the data was normalized using MAS5.0 software. Cells were identified post hoc as either developing retinal ganglion cells, amacrine cells or rod photoreceptor cells.

2008-06-16 | E-GEOD-9812 | biostudies-arrayexpress

Molecular heterogeneity of developing retinal ganglion and amacrine cells

Project description:During development of the central nervous system (CNS), cycling uncommitted progenitor cells give rise to a variety of distinct neuronal and glial cell types. As these different cell types are born, they progress from newly specified cells to fully differentiated neurons and glia. In order to define the developmental processes of individual cell types, single cell expression profiling was carried out on developing ganglion and amacrine cells of the murine retina. Individual cells from multiple developmental stages were isolated and profiled on Affymetrix oligonucleotide arrays. These experiments have yielded an expanded view of the processes underway in developing retinal ganglion and amacrine cells, as well as several hundred new marker genes for these cell types. In addition, this study has allowed for the definition of some of the molecular heterogeneity both between developing ganglion and amacrine cells and among subclasses of each cell type. Keywords: Single retinal cell gene expression profiling across multiple mouse developmental stages

2007-12-31 | GSE9812 | GEO

RNA-seq analysis of RNA from DBA/2J retinal ganglion cells

Project description:RNA-seq analysis from young and pre-glaucomatous DBA/2J retinal ganglion cells and control (age and sex-matched, D2-Gpnmb+) retinal ganglion cells

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Subcellular expression profiling of the growth cones of retinal ganglion cells (RGC)

Project description:Cue-directed axon guidance depends partly on local translation in growth cones. Many mRNA transcripts are known to reside in developing axons yet little is known about their subcellular distribution or, specifically, which transcripts are in growth cones. Laser capture microdissection (LCM) was used to isolate the growth cones of retinal ganglion cell (RGC) axons of two vertebrate species, mouse and Xenopus, coupled with unbiased genome-wide microarray profiling. Localized mRNA from the isolated growth cones of Xenopus laevis and Mus musculus retinal ganglion cells were subjected to microarray analysis

2010-11-23 | E-GEOD-25166 | biostudies-arrayexpress

RNA-Seq and ChIP-Seq gene profiling of retinal ganglion cells in Math5Cre-driven Knockout of Ezh2 and G9a in murine retinal ganglion cells at P0

Project description:Epigenetic mark deposition during embryonic development contribute to postnatal homeostasis and tissue stability. Previously, we found out that Ezh2 contributes critically to the function and postnatal cell survival in bipolar cells but not in retinal ganglion cells in the retina. (Yan et al. Postnatal onset of retinal degeneration by loss of embryonic Ezh2 repression of Six1. Sci Report. doi:10.1038/srep33887; (Cheng L, Wong LJ, Yan N, Han RC et al. Ezh2 does not mediate retinal ganglion cell homeostasis or their susceptibility to injury. PLoS One 2018;13(2):e0191853.). In this study, we used RNA-seq to define up- and down regulated genes in both Ezh2 and G9a deficient (Math5Cre; Ezh2f/fG9af/+; dKO) retinal ganglion cells (RGC) to evaluate the hypothesis of Ezh2 and G9a interaction that has been discussed in other tissues but in the retina. ChIP-Seq was applied to evaluate H3K27me3 histone marks in retinal ganglion cells in wild type (WT), Ezh2 (Math5Cre; Ezh2-/-, sKO) and combined G9a-Ezh2 (Math5Cre; G9a+/-Ezh2-/-, dKO) knockout mutant mice at P1.

2022-07-26 | GSE136360 | GEO

Retinal ganglion cells lipid profiling

Project description:The aim of this project was to characterize lipid profiles of the retinal ganglion cells (RGCs) with different regenerative capacity. RGCs were FACS-sorted (7,000 cells/sample) from mice of OPN4 Cre tdT and Thy1-CFP genotype. The treatment conditions included intact, optic nerve crush (ONC) and ONC plus CNTF to promote regeneration. Samples were then subject to lipid extraction and analyzed using LC-MS/MS, followed by identification and relative quantification in LipidSearch software.

2018-11-26 | ST001105 | MetabolomicsWorkbench

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