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Activation of AHR transcriptional activity upon treatment with indole-3-pyruvate

ABSTRACT: Activation of AHR transcriptional activity upon treatment with indole-3-pyruvate

PROVIDER: PRJNA599381 | ENA |

REPOSITORIES: ENA

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Activation of AHR transcriptional activity upon treatment with indole-3-pyruvate

Project description:Indole-3-pyruvate (I3P), an endogenous metabolite derived from tryptophan by gut microbiota and IL4I1 enzyme in humans can potentially activate the transcriptional activity of the Aryl Hydrocarbon receptor. Here we test this by stimulating AHR proficient U-87MG cells with I3P alone or in combination with the AHR antagonist SR1.

2020-08-19 | GSE143240 | GEO

Analysis of HeLa cell transcriptome changes induced by Indole-3-pyruvate and mIL4i1

Project description:We analyzed the mRNA changes induced by Indole-3-pyruvate (I3P) and WT or K351A mutant mIL4i1 in HeLa cells.

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Analysis of THP-1 cell transcriptome changes induced by Phenylpyruvate, 4-Hydroxyphenylpyruvate and Indole-3-pyruvate

Project description:We analyzed the mRNA changes iduced by the alpha keto acids Phenylpyruvate (PP), 4-Hydroxyphenylpyruvate (4HPP) or Indole-3-pyruvate (I3P) in THP-1 cells.

2021-03-07 | GSE161159 | GEO

Proteomic characterization of the cellular effects of AHR activation by microbial tryptophan catabolites in endotoxin-activated human macrophages

Project description:Sensing of microbial tryptophan catabolites by the aryl hydrocarbon receptor (AhR) plays a pivotal role in host-microbiome homeostasis by modulating the host immune response. Thereby the involved cellular processes triggered by the metabolites are largely unknown. We analyzed proteomic changes in macrophages trough 24h after treatment with the tryptophan metabolites indole-3-acetic acid (I3AA) or indole-3-aldehyde (IAld), as well as the prototypic AhR-ligand Benzo(a)pyrene (BaP) in the absence and presence of LPS to identify affected processes and pathways.

2021-11-03 | PXD025022 | Pride

Analysis of HeLa cell transcriptome changes induced by Indole-3-pyruvate and mIL4i1

Project description:Analysis of HeLa cell transcriptome changes induced by Indole-3-pyruvate and mIL4i1

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AHR activation in glioblastoma cells [I3P_HPP_PP_U87_U87]

Project description:The objective of this study was to analyze AHR activation through aromatic amino acid metabolism. To this end, glioblastoma cells were exposed to aromatic amino acid derived metabolites and their ability to activate AHR was analysed. In addition, AHR activation was evaluated in glioblastoma cells expressing IL4I1, an aromatic amino acid degrading enzyme, with or without shRNA mediated knockdown of AHR.

2020-08-19 | GSE129976 | GEO

AHR activation in glioblastoma cells [I3CA_U87]

2020-08-19 | GSE129974 | GEO

AHR activation in glioblastoma cells [FICZ_KynA]

2020-08-19 | GSE129972 | GEO

AHR activation in glioblastoma cells [IL4I1_shAHR_U87]

2020-08-19 | GSE129977 | GEO

Activation of AhR promotes phosphorylation of ARNT isoform 1 as a switch for optimal AhR activity in human T cell malignancies.

Project description:The aryl hydrocarbon receptor nuclear translocator (ARNT) is a transcription factor present in immune cells as a long and short isoform, referred to as isoform 1 and 3, respectively. However, investigation into potential ARNT isoform-specific immune functions is lacking despite the well-established heterodimerization requirement of ARNT with, and for the activity of, the aryl hydrocarbon receptor (AhR), a critical mediator of immune homeostasis. Here, using global and targeted transcriptomics analyses we show that the relative ARNT isoform 1:3 ratio in human T cell lymphoma cells dictates the amplitude and direction of AhR target gene regulation. Specifically, shifting the ARNT isoform 1:3 ratio lower by suppressing isoform 1 enhances, or higher by suppressing isoform 3 abrogates AhR responsiveness to ligand activation through preprograming a cellular genetic background that directs explicit gene expression patterns. Moreover, the fluctuations in gene expression patterns that accompany a decrease or increase in the ARNT isoform 1:3 ratio are associated with inflammation or immunosuppression, respectively. Molecular studies identified the unique casein kinase 2 (CK2) phosphorylation site within isoform 1 as an essential parameter to the mechanism of ARNT isoform-specific regulation of AhR signaling. Notably, CK2-mediated phosphorylation of ARNT isoform 1 is dependent on ligand-induced AhR nuclear translocation and is required for optimal AhR target gene regulation. These observations reveal ARNT as a central modulator of AhR activity predicated on the status of the ARNT isoform ratio and suggest that ARNT-based therapies are a viable option for tuning the immune system to target immune disorders.

2022-03-31 | GSE184151 | GEO

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