Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Widely distributed neurotensinergic neurons in the brainstem regulate NREM sleep in mice

ABSTRACT: Widely distributed neurotensinergic neurons in the brainstem regulate NREM sleep in mice

PROVIDER: PRJNA599587 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Widely distributed neurotensinergic neurons in the brainstem regulate NREM sleep in mice

Project description:Microarray identifies enriched expression of Nts in SubLDT, compared to dorsal and ventral area

2020-01-09 | GSE143280 | GEO

An Excitatory Circuit in Perioculomotor Midbrain for Non-REM Sleep Control

Project description:The perioculomotor (pIII) region of the midbrain was postulated as a sleep-regulating center in the 1890s but largely neglected in subsequent studies. Using activity-dependent labeling and gene-expression profiling, we identified pIII neurons that promote non-REM (NREM) sleep.

2019-05-01 | GSE124681 | GEO

Orexin/Hypocretin Receptor 2 Signaling in MCH Neurons Regulates REM sleep and Insulin Sensitivity

Project description:Orexin/hypocretin receptor type 2 (Ox2R), which is widely expressed in the brain, receives orexin signals and modulates sleep and metabolism. Ox2R selective agonists are currently under clinical trials for narcolepsy treatment. Nevertheless, it remains unclear whether Ox2R exerts an inhibitory effect via Gi proteins in addition to an excitatory effect via Gq proteins. Here, we focused on Ox2R expression and function in MCH neurons, which have opposite roles to orexin neurons in sleep and metabolism regulation. Ox2R-expressing MCH neurons showed heterogeneity of RNA expression, and orexin B application in brain slices induced both excitatory and inhibitory responses in distinct MCH neuron populations. Ox2R inactivation in MCH neurons reduced transitions from NREM to REM sleep and impaired insulin sensitivity with hyperphagia. In conclusion, Ox2R mediates excitatory and inhibitory responses in MCH neuron subpopulations in vivo, which might regulate sleep and metabolism.

2024-11-19 | GSE281229 | GEO

Single-cell transcriptomics and analysis for new molecular regulators of sleep

Project description:The molecular mechanisms governing sleep are largely unknown. Here, we used a combination of single-cell RNA sequencing to interrogate the molecular and functional underpinnings of sleep. Different cell types in three important brain regions for sleep (brainstem, cortex and hypothalamus) had a similar transcriptional response to sleep need, with a large proportion of cells changing during recovery sleep. In contrast, sleep deprivation regulated expression of different functions in each brain region. This includes antigen processing, synaptic transmission and cellular metabolism in brainstem, cortex and hypothalamus, respectively. Increased sleep need enhances expression of the transcription factors Sox2, Mafb, and Zic1 in brainstem; Hlf, Cebpb and Sox9 in cortex, and Atf3, Fosb and Mef2c in hypothalamus. In turn, these transcription factors regulate downstream gene expression during sleep deprivation and recovery. In cortex, we also interrogated the proteome of two major cell types: neurons and astrocytes. We found surprising functional overlap of proteins that mediate vesicle and neurotransmitter transport in both cell types. In contrast, other functions were specific to each cell type.

2022-08-24 | GSE137665 | GEO

Single-cell transcriptomics and cell-specific proteomics reveals new molecular regulators of sleep

Project description:Every day, we sleep for a third of the day. Sleep is important for cognition, brain waste clearance, metabolism, and immune responses. Homeostatic regulation of sleep is maintained by progressively rising sleep need during wakefulness, which then dissipates during sleep. The molecular mechanisms governing sleep are largely unknown. Here, we used a combination of single-cell RNA sequencing and cell-type specific proteomics to interrogate the molecular and functional underpinnings of sleep. Different cell-types in the brain regions show similar transcriptional response to sleep need whereas sleep deprivation changes overall expression indicative of altered antigen processing, synaptic transmission and cellular metabolism in brainstem, cortex and hypothalamus, respectively. Increased sleep need enhances expression of transcription factor Sox2, Mafb, and Zic1 in brainstem; Hlf, Cebpb and Sox9 in cortex, and Atf3, Fosb and Mef2c in hypothalamus. Results from cell-type proteome analysis suggest that sleep deprivation changes abundance of proteins in cortical neurons indicative of altered synaptic vesicle cycles and glucose metabolism whereas in astrocytes it alters the abundance of proteins associated with fatty acid degradation. Similarly, phosphoproteomics of each cell type demonstrates large shifts in site-specific protein phosphorylation in neurons and astrocytes of sleep deprived mice. Our results indicate that sleep deprivation regulates transcriptional, translational and post-translational responses in a cell-specific manner and advances our understanding of the cellular and molecular mechanisms that govern sleep-wake homeostasis in mammals.

2022-10-13 | PXD018334 | Pride

Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner [agilent_forebrain]

Project description:Micro-RNAs (miRNAs) are key, post-transcriptional regulators of gene expression that have been implicated in a plethora of biological processes. Their role in sleep, however, has received little attention. We first investigated the effects of sleep deprivation on the brain miRNA transcriptome. Sleep deprivation affected 52 miRNAs in the whole brain, and particularly miR-709, an activity-dependent microRNA, enriched in the cerebral cortex.

2024-01-12 | GSE189348 | GEO

Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner [agilent_cortex_hippocampus]

Project description:Micro-RNAs (miRNAs) are key, post-transcriptional regulators of gene expression that have been implicated in a plethora of biological processes. Their role in sleep, however, has received little attention. In this experiment we investigated the effects of sleep deprivation on the cortex and hippocampus miRNA transcriptome. In this experiment we observed different miRNAs being affected by sleep deprivation in the cortex and hippocampus.

2024-01-12 | GSE189319 | GEO

Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner

Project description:Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner

| PRJNA782625 | ENA

Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner [agilent_forebrain]

Project description:Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner [agilent_forebrain]

| PRJNA782634 | ENA

Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner [NanoString]

Project description:Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner [NanoString]

| PRJNA782534 | ENA