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ABSTRACT: Acinetobacter baumannii abaI/abaR quorum sensing system

PROVIDER: PRJNA600672 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10878040_1.fastq.gz	Fastqsanger.gz
	SRR10878040_2.fastq.gz	Fastqsanger.gz
	SRR10878041_1.fastq.gz	Fastqsanger.gz
	SRR10878041_2.fastq.gz	Fastqsanger.gz
	SRR10878042_1.fastq.gz	Fastqsanger.gz

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Transcriptome profiling of wild type and ΔabaI::Km mutant

Project description:Transcriptome profiling of quorum sensing regulated genes in Acinetobacter baumannii 863

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The abaI/abaR quorum sensing system effects pathogenicity in Acinetobacter baumannii

Project description:Acinetobacter baumannii is a Gram-negative pathogen that has emerged as one of the most troublesome pathogens for health care institutions globally. Bacterial quorum sensing (QS) is a process of cell-to-cell communication that relies on the production, secretion and detection of autoinducer (AI) signals to share information about cell density and regulate gene expression accordingly. The molecular and genetic basis of Acinetobacter baumannii virulence remains poorly understood. Therefore, the contribution of the abaI/abaR quorum sensing system to growth characteristics, morphology, biofilm formation, resistance, motility and virulence of Acinetobacter baumannii was studied in detail. RNA-seq analysis indicated that genes involved in various aspects of energy production and conversion, Valine, leucine and isoleucine degradation and lipid transport and metabolism are associated with bacterial pathogenicity. Our work provides a new insight into abaI/abaR quorum sensing system effects pathogenicity in A. baumannii. We propose that targeting the AHL synthase enzyme abaI could provide an effective strategy for attenuating virulence. On the contrary, interdicting the autoinducer synthase–receptor abaR elicits unpredictable consequences, which may lead to enhanced bacterial virulence.

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Project description:Acinetobacter baumannii A1S_1874 gene encodes as a LysR-type transcriptional regulator. LysR family regulators known to regulate biofilm formation, antibiotic resistance, and the expression of diverse genes in other Gram-negative bacteria. However, A1S-1874 has never been characterized in Acinetobacter baumannii, and the studies about the regulon of A1S-1874 are not discovered. In this study we revealed that A1S_1874 differentially regulates at least 302 genes including the csu pilus operon, N-acylhomoserine lactone synthese gene, A1S_0112-A1S_0118 operon, type 1v secretion system related genes that are involved in biofilm formation, surface motility, adherence, quorum sensing and virulence. Overall, our data suggests that A1S-1874 is a key regulator of Acinetobacter baumannii biofilm formation and gene expression.

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Transcriptional profiling by RNA-seq of A. baumannii AB5075 wild-type, abaI::Tn26 and ∆abaM::Tn26 QS mutants.

Project description:Acinetobacter baumannii possesses a single divergent luxR/luxI-type quorum sensing (QS) locus named abaR/abaI. This locus also contains a third gene located between abaR and abaI which we term abaM that codes for an uncharacterized member of the RsaM protein family known to regulate N-acylhomoserine lactone (AHL) dependent QS and the expression of diverse genes in other β- and γ-proteobacteria. However, abaM has never been charatcerized in Acinetobacter baumannii, and the studies about the regulon of abaI are limited. In this study we revealed that AbaM differentially regulates at least 76 genes including the csu pilus operon and the acinetin 505 lipopeptide biosynthetic operon, that are involved in surface adherence, biofilm formation and virulence. A comparison of the wild type, abaM::Tn26 and abaI::Tn26 transcriptomes, indicates that AbaM regulates ~21% of the QS regulon including the csu operon. Moreover, the QS genes (abaI/abaR) were among the most upregulated in the abaM::Tn26 mutant. Overall, our data suggests that abaM is a key regulator of Acinetobacter baumannii QS and gene expression.

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Project description:Quorum sensing system-regulated protein affect the spoilage potential of co-culturing Acinetobacter johnsonii and Pseudomonas fluorescens from spoilage bigeye tuna (Thunnus obesus) by proteomics

2020-04-18 | PXD018646 |

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Project description:Quorum Sensing/Quorum Quenching in Acinetobacter baumannii clinical strains

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The abaI/abaR quorum sensing system effects pathogenicity in Acinetobacter baumannii

Project description:The abaI/abaR quorum sensing system effects pathogenicity in Acinetobacter baumannii

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2020-06-15 | GSE148264 | GEO

Combined analysis of genome-wide DNA methylome and transcriptome reveals the first epigenetic-based antibiotic-resistance mechanism in Acinetobacter baumannii

Project description:Using Nanopore sequencing, our study has revealed a close correlation between genomic methylation levels and antibiotic resistance rates in Acinetobacter Baumannii. Specifically, the combined genome-wide DNA methylome and transcriptome analysis revealed the first epigenetic-based antibiotic-resistance mechanism in A. baumannii. Our findings suggest that the precise location of methylation sites along the chromosome could provide new diagnostic markers and drug targets to improve the management of multidrug-resistant A. baumannii infections.

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3D-printed wound dressings containing a fosmidomycin-derivative prevent Acinetobacter baumannii biofilm formation in vitro and in vivo

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