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RNA-seq of SI adenoma tissue from ApcMin and ApcMin-PCdel Mice

ABSTRACT: RNA-seq of SI adenoma tissue from ApcMin and ApcMin-PCdel Mice

PROVIDER: PRJNA600750 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10874365.fastq.gz	Fastqsanger.gz
	SRR10874366.fastq.gz	Fastqsanger.gz
	SRR10874367.fastq.gz	Fastqsanger.gz
	SRR10874368.fastq.gz	Fastqsanger.gz
	SRR10874369.fastq.gz	Fastqsanger.gz

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Single cell changes in RSPO1-Fc treated intestinal adenoma organoids from ApcMin/+ mice

Project description:We constructed AAV-vectors for systemic expression of a soluble RSPO1 protein in ApcMin/+ mice. We found that the RSPO1-Fc fusion protein suppresses the Wnt/ß-catenin signaling activity in intestinal adenomas and in adenoma-derived intestinal organoids ex vivo, but not in normal intestinal epithelial cells. In the Apc mutant cells, the RSPO1-Fc fusion protein activated the TGFß/SMAD signaling pathway to suppress several Wnt target genes and adenoma growth, which effect was rescued suppressed by the TGFß receptor kinase inhibitor SB-431542. Simultaneously, RSPO1-Fc induced proliferation of the normal intestinal stem cells, giving them a growth advantage over the mutant cells, which enabled the intestinal epithelium to eventually outgrow the adenoma cells. Prolonged systemic expression of AAV-RSPO1-Fc decreased significantly the number of the intestinal adenomas and improved the overall survival of ApcMin/+ mice. Thus RSPO1-Fc provides the normal intestinal epithelial cells a growth advantage when compared to the adenoma cells, which eventually leads to the extrusion of the adenomatous tissue. An attractive idea now is to exploit such differential response of normal vs. cancer cells in cancer therapy.

2020-02-29 | GSE146099 | GEO

Single cell changes in AAV-Ctrl or AAV-RSPO1-Fc treated intestinal adenoma cells from ApcMin/+ mice

2020-03-01 | GSE146138 | GEO

RNA-seq of SI adenoma tissue from ApcMin and ApcMin-PCdel Mice

Project description:Adenomas derived from PCdel mice exhibited reduced expression of paneth cell marker genes as well as Wnt3.

2020-12-20 | GSE143487 | GEO

let-7e downregulation characterizes early phase colonic adenoma in APCMin/+ mice and human FAP patients

Project description:The crypt-villus axis represents the essential unit of the small intestine, which integrity and functions are fundamental to assure tissue and whole-body homeostasis. Disruption of pathways regulating the fine balance between proliferation and differentiation results in diseases development. Nowadays, it is well established that microRNAs (miRNAs) play a crucial role in the homeostasis maintenance and perturbation of their levels may promote tumor development. Here, by using microarray technology, we analysed the miRNAs differentially expressed between the crypt and the villus in mice ileum. The emerged miRNAs were further validated by Real Time qPCR in mouse model (ApcMin/+), human cell lines and human tissue samples (FAP) of colorectal cancer (CRC). Our results indicated that miRNAs more expressed in the villi compartment are negatively regulated in tumor specimens, thus suggesting a close association between these microRNAs and the differentiation process. Particularly, from our analysis let-7e appeared to be a promising target for possible future therapies and a valuable marker for tumor staging, being upregulated in differentiated cells and downregulated in early stage colonic adenoma samples.

2021-03-17 | GSE165991 | GEO

Single cell changes in RSPO1-Fc treated intestinal adenoma organoids from ApcMin/+ mice

Project description:Single cell changes in RSPO1-Fc treated intestinal adenoma organoids from ApcMin/+ mice

| PRJNA609283 | ENA

Single-cell analysis of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin intestinal adenoma cells at the age of 11 wks

Project description:Comparison of Villin-Cre;Apc min;Lef1 fl/fl (VApcMinL) and ApcMin adenoma cells at the age of 11 wks. 2-3 male mice and 3-10 tumors per group were used in the experiment. Experiment was repeated twice (ApcMin2 and VApcMinL2 samples).

2021-12-10 | GSE174097 | GEO

CSF1R mediates effects of miR-34a loss during intestinal tumorigenesis in ApcMin mice (adenoma)

Project description:CSF1R mediates effects of miR-34a loss during intestinal tumorigenesis in ApcMin mice (adenoma)

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Expression data from cav1-/+apcmin/+ mouse intestinal tissue

Project description:We used microarrays to decipher the global programme of gene expression in normal colon tissue versus colorectal tumor tissue explants from 6 months old cav1-/+ apcmin/+ mice and identified distinct classes of differentially regulated genes.

2019-01-10 | GSE124838 | GEO

let-7e downregulation characterizes early phase colonic adenoma in APCMin/+ mice and human FAP patients

Project description:let-7e downregulation characterizes early phase colonic adenoma in APCMin/+ mice and human FAP patients

| PRJNA698939 | ENA

CSF1R mediates effects of miR-34a loss during intestinal tumorigenesis in ApcMin mice (adenoma)

Project description:To address the in vivo relevance of CSF1R regulation by miR-34a during intestinal tumor formation, we generated ApcMin/+ mice with intestinal-epithelial cell (IEC)-specific deletions of the Mir34a and/or Csf1r genes. The mRNA expression profiles of intestinal adenomas with and without functional miR-34a and/or Csf1r were compared.

2022-10-04 | GSE167448 | GEO

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