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TRIM3 depletion in MCF-7 cells

ABSTRACT: TRIM3 depletion in MCF-7 cells

PROVIDER: PRJNA601105 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10883676.fastq.gz	Fastqsanger.gz
	SRR10883677.fastq.gz	Fastqsanger.gz
	SRR10883678.fastq.gz	Fastqsanger.gz
	SRR10883679.fastq.gz	Fastqsanger.gz
	SRR10883680.fastq.gz	Fastqsanger.gz

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Project description:In previous study, we found circFAT1(e2) was highly expressed in breast cancer tissues and cell lines. And this factor is also a crucial role in breast cancer genesis and development. So in this study, we knocked down circFAT1(e2) in MCF-7 cells. The viability and metastasis was significantly inhibited with circFAT1(e2) depletion. In order to explore the potential down-stream gene of circFAT1(e2) in breast cancer cells. We extracted the total RNA from circFAT1(e2) knockdown MCF-7 cells and negative control group by using TriZol reagent. Then, toal RNA was subjected to transcriptome sequencing to determine the potential down-stream targets of circFAT1(e2). We expect to reveal the underlied mechanism of the regulatory effect on breast cancer of circFAT1(e2).

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