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Vitis vinifera

ABSTRACT: Vitis vinifera root miRNA sequencing.

PROVIDER: PRJNA601829 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10906903.fastq.gz	Fastqsanger.gz
	SRR10906904.fastq.gz	Fastqsanger.gz
	SRR10906905.fastq.gz	Fastqsanger.gz
	SRR10906906.fastq.gz	Fastqsanger.gz

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Project description:Vitis vinifera RNA degradome Isolated polyadenylated RNA from total RNA extracts of Vitis vinifera leaves, were ligated to 5'-adapter that include san MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel and to a 3'- dsDNA adapter and PCR amplified. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

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