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Next Generation Sequencing Analysis of Transcriptomes of the HEK293T-Cas9 cells transfected with plasmid containing programmed scaffold RNA

ABSTRACT: Next Generation Sequencing Analysis of Transcriptomes of the HEK293T-Cas9 cells transfected with plasmid containing programmed scaffold RNA

PROVIDER: PRJNA601968 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10908735_1.fastq.gz	Fastqsanger.gz
	SRR10908735_2.fastq.gz	Fastqsanger.gz
	SRR10908736_1.fastq.gz	Fastqsanger.gz
	SRR10908736_2.fastq.gz	Fastqsanger.gz
	SRR10908737_1.fastq.gz	Fastqsanger.gz

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Project description:By performing RNA sequencing (RNA-seq) analysis on the HEK293T-Cas9 cells which transfected with plasmid containing programmed scaffold RNA for non-targeting or for targeting Hemoglobin subunit gamma-1 (HBG1) we want to investigate whether the scaffold RNA and the endogenous mRNA could be captured in regular reverse transcription reaction. RNA-seq analysis showed that we can capture the scaffold RNA together with the endogenous mRNA precisely.

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