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Differential transcriptional expression by Babesia bovis during parasite development within bovine erythrocytes and tick hemolymph

ABSTRACT: Differential transcriptional expression by Babesia bovis during parasite development within bovine erythrocytes and tick hemolymph

PROVIDER: PRJNA602683 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10951049_1.fastq.gz	Fastqsanger.gz
	SRR10951049_2.fastq.gz	Fastqsanger.gz
	SRR10951050_1.fastq.gz	Fastqsanger.gz
	SRR10951050_2.fastq.gz	Fastqsanger.gz
	SRR10951051_1.fastq.gz	Fastqsanger.gz

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Differential transcriptional expression by Babesia bovis during parasite development within bovine erythrocytes and tick hemolymph

Project description:Babesia parasites transition between a mammalian host, where they cause babesiosis, and the tick vector that transmits them. This transition provides an environmental signal resulting in altered gene expression allowing the completion of the parasite’s life cycle. A comparison of the different life stages that occur within mammalian and tick hosts can provide insight into the adaptation of Babesia to these different environments. In this study, we used RNA-Seq to compare gene expression between Babesia bovis blood stages and tick derived kinetes.

2020-10-21 | GSE144066 | GEO

Comparison of high throughput RNA sequences between Babesia bigemina and Babesia bovis revealed consistent differential gene expression that is required for Babesia life cycle in the vertebrate and invertebrate hosts.

Project description:To understand Babesia gene regulation during tick and mammalian host infection, we performed high throughput RNA-sequencing using samples collected from calves and Rhipicephalus microplus ticks infected with Babesia bigemina. We evaluated gene expression differences between B. bigemina kinetes and blood-stage parasites

2022-12-20 | GSE214115 | GEO

Rhipicephalus microplushemocyte innate cellular and humoral immune responses to babesialinfection

Project description:To understand the effect of Babesia infection on Rhipicephalus microplus hemocyte gene expression, we performed high throughput RNA-sequencing using samples collected from Rhipicephalus microplus uninfected tick hemolymph and infected with either Babesia bigemina or Babesia bovis. We evaluated gene expression differences that may be attributed to tick immune defense to babesial infection.

2023-12-31 | GSE243493 | GEO

Babesia bovis

Project description:Babesia bovis

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Comparative transcriptome analysis of geographically distinct virulent and attenuated Babesia bovis

Project description:Geographical distinct virulent Babesia bovis strains have similar gene expression changes as they go through attenuation. Pair end RNA-sequencing reads on three biological replicate sample pairs of virulent parent and attenuated derivative Babesia bovis strain isolated in Argentina.

2013-10-23 | E-GEOD-51560 | biostudies-arrayexpress

Comparative transcriptome analysis of geographically distinct virulent and attenuated Babesia bovis

Project description:Geographical distinct virulent Babesia bovis strains have similar gene expression changes as they go through attenuation.

2013-10-23 | GSE51560 | GEO

Morphological and molecular descriptors of the developmental cycle of Babesia divergens parasites in human erythrocytes

Project description:Human babesiosis, especially caused by the cattle derived Babesia divergens parasite, is on the increase, resulting in renewed attentiveness to this potentially life threatening emerging zoonotic disease. The molecular mechanisms underlying the pathophysiology and intra-erythrocytic development of these parasites are poorly understood. This impedes concerted efforts aimed at the discovery of novel anti-babesiacidal agents. By applying sensitive cell biological and molecular functional genomics tools, we describe the intra-erythrocytic development cycle of B. divergens parasites from immature, mono-nucleated ring forms to bi-nucleated paired piriforms and ultimately multi-nucleated tetrads that characterizes zoonotic Babesia spp. This is further correlated for the first time to nuclear content increases during intra-erythrocytic development progression, providing insight into the part of the life cycle that occurs during human infection. High-content temporal evaluation elucidated the contribution of the different stages to life cycle progression. Moreover, molecular descriptors indicate that B. divergens parasites employ physiological adaptation to in vitro cultivation. Additionally, differential expression is observed as the parasite equilibrates its developmental stages during its life cycle. Together, this information provides the first temporal evaluation of the functional transcriptome of B. divergens parasites; information that could be useful in identifying biological processes essential to parasite survival for future anti-babesiacidal discoveries. Two-condition experiment, Untreated vs.Treated B. divergens parasites, cultured in human erythrocytes. Treatment with a piperidinyl-benzimidizalone analogue. Biological replicates: 3 untreated (control) replicates, 3 treated replicates. The 6-sample dataset represents untreated(control) vs pooled_reference samples at various timepoints.

2015-04-10 | E-GEOD-67677 | biostudies-arrayexpress

Babesia bovis

Project description:EST sequencing project for Babesia bovis

| PRJNA152 | ENA

Babesia bovis

Project description:Comparative transcriptome analysis of geographically distinct virulent and attenuated Babesia bovis

| PRJNA223413 | ENA

Tick passage results in enhanced attenuation of Babesia bovis.

Project description:Serial blood passage of virulent Babesia bovis in splenectomized cattle results in attenuated derivatives that do not cause neurologic disease. Tick transmissibility can be lost with attenuation, but when retained, attenuated B. bovis can revert to virulence following tick passage. This study provides data showing that tick passage of the partially attenuated B. bovis T2Bo derivative strain further decreased virulence compared with intravenous inoculation of the same strain in infected animals. Ticks that acquired virulent or attenuated parasites by feeding on infected cattle were transmission fed on naive, splenectomized animals. While there was no significant difference between groups in the number of parasites in the midgut, hemolymph, or eggs of replete female ticks after acquisition feeding, animals infected with the attenuated parasites after tick transmission showed no clinical signs of babesiosis, unlike those receiving intravenous challenge with the same attenuated strain prior to tick passage. Additionally, there were significantly fewer parasites in blood and tissues of animals infected with tick-passaged attenuated parasites. Sequencing analysis of select B. bovis genes before and after tick passage showed significant differences in parasite genotypes in both peripheral blood and cerebral samples. These results provide evidence that not only is tick transmissibility retained by the attenuated T2Bo strain, but also it results in enhanced attenuation and is accompanied by expansion of parasite subpopulations during tick passage that may be associated with the change in disease phenotype.

| S-EPMC4187863 | biostudies-other

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