Project description:To provide a more detailed insight into molecular changes in seeds exposed to the accelerated ageing process, we excised embryos and compared the differences in the proteome composition. Seeds collected from four contrasting locations were exposed to accelerated ageing. Next, embryos were dissected and analyzed in five biological replicates [1-5]. Sample description:CAS [1-5] - location n. 5 - (Caslav) - high yield, above average temperature and high rainfall; a significant decrease in germination energy after the accelerated ageing test. UHO [1-5] - location n. 11 (Uhersky Ostroh) - low yield, high temperature and low rainfall; very low decrease in germination energy after the accelerated ageing test.CH [1-5] - location n. 2 (Chrastava) - average yield, low temperature, very high rainfall; a significant decrease in germination energy after the accelerated ageing test.K [1-5] - location n. 8 (Krasne Udoli) - average yield, low temperature, average rainfall; a significant decrease in germination energy after the accelerated ageing test.
Project description:Greater change in arbuscular mycorrhizal fungal richness as a response to short-term rainfall exclusion across the North American Monsoon season
Project description:Transcriptional profiling of Ovine skin samples comparing resistant (RES) to susceptible (SUS) animals following fleece rot induction. Two condition experiment, RES vs SUS biopsies, over 3 time points. 31 RES 31 SUS T0: Prior to commencment of treatment T1: 2-3 days post commencment T2: 11 days post commencment The first skin biopsies were taken on the first day (T0) prior to commencement of the five day regime of simulated rainfall. Subsequent skin biopsies were taken 2-3 days into the wetting regime (T1) and following a recovery period of 11 days from the time that the wetting regime had begun (T2).