Project description:To investigate the biological role of Gdown1, we chose mouse liver and generated Gdown1 conditional knockout mice. Differentially expressed genes in Gdown1 KO liver were analyzed by microarrays.
Project description:To investigate the biological role of Gdown1, differentially expressed genes in Gdown1 knockout and p53/Gdown1 double-knockout mouse liver were analyzed by RNA-seq.
Project description:We applied DIA Proteomic Analysis to explore Differentially expressed proteins between WT sperms and Cfap77-KO sperms. In this DIA Proteomic Analysis project, there were 6 Mus_musculus samples named W1,W2,W3,C1,C2,C3 and we did 1 technical duplicate experiments. Totally 94339 peptides and 6525 proteins were identified with 1% FDR. Proteins with 2 fold change (mean value of all comparision groups) and P-value (t-test of all comparision groups) less than 0.05 were defined as differentially expressed proteins.
Project description:We applied iTRAQ Quantification Proteomics to explore Differentially expressed proteins between WT testis and Cfap70-KO testis. In this iTRAQ quantification project,there were 6 Mus_musculus samples named C_1, C_2, C_3, K_1, K_2, K_3 and we did 1 technical duplicate experiments. Totally 480850 spectrums were generated, 38748 peptides and 6734 proteins were identified with 1% FDR. proteins with 1.2 fold change(mean value of all comparison groups) and P-value(t-test of all comparision groups) less than 0.05 were defined as differentially expressed proteins.
Project description:Differentially expressed genes of CD11b+Gr-1+ immature myeloid cells (IMCs) in the bone marrow and colonic tumor setting of histidine decarboxylase (HDC)-KO mice were examined by microarray (Affymetrix Mouse 430.2 array). Myeloid differentiation-related candidate genes were sought to be isolated and functionally studied.