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Drosera rotundifolia induces gene expression changes in human bronchial epithelial cells 16HBE

ABSTRACT: Drosera rotundifolia induces gene expression changes in human bronchial epithelial cells 16HBE

PROVIDER: PRJNA603072 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10961644.fastq.gz	Fastqsanger.gz
	SRR10961645.fastq.gz	Fastqsanger.gz
	SRR10961646.fastq.gz	Fastqsanger.gz
	SRR10961647.fastq.gz	Fastqsanger.gz
	SRR10961648.fastq.gz	Fastqsanger.gz

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Drosera rotundifolia induces gene expression changes in human bronchial epithelial cells 16HBE

Project description:16HBE cells were incubated for 3 or 6h with a homeopathic preparation of Drosera r. or the solvent control and the transcriptome was analyzed by RNA sequencing. The expression changes of the main statistically significant genes were validated through follow-up experiments using RT-qPCR. Compared with the control solution, Drosera r. changed the expression of dozens of genes already after 3h and this effect was amplified after 6 hours of treatment. The main target genes were some ligands of epithelial growth factor receptor (EREG, AREG, EPGN), genes involved in xenobiotic detoxification (CYP1B1, TIPARP) and chemokines. The network of the main biological functions included epithelial cell proliferation, regulation of angiogenesis, cell chemotaxis, and wound healing. Drosera r. acts on a complex and faceted set of genes, potentially involved in different layers of the bronchial mucosa. The global effect of the plant in 16HBE epithelial cells is a mild stress response that primes the epithelial reparative process and recruits the innate cell defense.

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Project description:Drosera rotundifolia has been traditionally used for the treatment of respiratory diseases in phytotherapy and homeopathy. The mechanisms of action recognized so far are linked to the known effects of specific components, such as flavonoids, but are not completely understood. In this study, the biological functions of D. rotundifolia were explored in vitro following the treatment of bronchial epithelial cells, which are the potential targets of the pharmacological effects of the herbal medicine. To do so, the whole plant ethanolic extract was 1000-fold diluted in water (D. rotundifolia 3×) and added to a 16HBE human cell line culture for 3 h or 6 h. The effects on gene expression of the treatments and corresponding controls were then investigated by RNA sequencing. The differentially expressed genes were validated through RT-qPCR, and the enriched biological functions involved in the effects of treatment were investigated. D. rotundifolia 3×?did not impair cell viability and was shown to be a stimulant of cell functions by regulating the expression of dozens of genes after 3 h, and the effects were amplified after 6 h of treatment. The main differentially expressed genes encoded ligands of epithelial growth factor receptor, proteins involved in xenobiotic detoxification and cytokines, suggesting that D. rotundifolia 3×?could stimulate self-repair systems, which are impaired in airway diseases. Furthermore, D. rotundifolia 3×?acts on a complex and multifaceted set of genes and may potentially affect different layers of the bronchial mucosa.

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