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Comprehensive Expression Analysis of mRNA and microRNA for Investigation of Compensatory Mechanisms in the Rat Kidney after Unilateral Nephrectomy (Male_mRNA)

ABSTRACT: Comprehensive Expression Analysis of mRNA and microRNA for Investigation of Compensatory Mechanisms in the Rat Kidney after Unilateral Nephrectomy (Male_mRNA)

PROVIDER: PRJNA603238 | ENA |

REPOSITORIES: ENA

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Comprehensive Expression Analysis of mRNA and microRNA for Investigation of Compensatory Mechanisms in the Rat Kidney after Unilateral Nephrectomy

Project description:Comprehensive Expression Analysis of mRNA and microRNA for Investigation of Compensatory Mechanisms in the Rat Kidney after Unilateral Nephrectomy

| PRJNA603235 | ENA

Comprehensive Expression Analysis of mRNA and microRNA for Investigation of Compensatory Mechanisms in the Rat Kidney after Unilateral Nephrectomy (Female_mRNA)

Project description:Comprehensive Expression Analysis of mRNA and microRNA for Investigation of Compensatory Mechanisms in the Rat Kidney after Unilateral Nephrectomy (Female_mRNA)

| PRJNA603237 | ENA

Comprehensive Expression Analysis of mRNA and microRNA for Investigation of Compensatory Mechanisms in the Rat Kidney after Unilateral Nephrectomy (Male_miRNA)

Project description:Comprehensive Expression Analysis of mRNA and microRNA for Investigation of Compensatory Mechanisms in the Rat Kidney after Unilateral Nephrectomy (Male_miRNA)

| PRJNA603239 | ENA

Comprehensive Expression Analysis of mRNA and microRNA for Investigation of Compensatory Mechanisms in the Rat Kidney after Unilateral Nephrectomy (Female_mRNA)

Project description:Compensation is a physiological response that occurs during chemical exposure to maintain homeostasis. Because compensatory responses are not usually considered adverse effects, it is important to understand compensatory mechanisms for chemical risk assessment. Although the kidney is a major target organ for toxicity, there is controversy over whether hyperplasia or hypertrophy contributes to the compensatory mechanism, and there is limited information to apply for chemical risk assessment. In the current study, compensatory mechanisms of the kidney were investigated in a unilateral nephrectomy (UNx) model using adult male and female rats. In residual kidneys of male and female rats after UNx, 5-bromo-2'-deoxyuridine-labeling indices and mRNA expression of cell cycle-related genes were increased, although there were no fluctuations in mRNA expression of transforming growth factor-β1, which contributes to hypertrophy in renal tubules. Pathway analysis using mRNA expression data from a cDNA microarray revealed that canonical pathways related to cell proliferation were mainly activated and that forkhead box M1 (FOXM1) was an upstream regulator of compensatory cell proliferation in residual kidneys of male and female rats. cDNA microarray for microRNAs (miRNAs) demonstrated that 9 miRNAs were downregulated in residual kidneys, and mRNA/miRNA integrated analysis indicated that miRNAs were associated with the expression of factors downstream of FOXM1. Overall, these results suggested that FOXM1-mediated hyperplasia rather than hypertrophy contributed to compensatory mechanisms in the kidney and that miRNAs regulated downstream FOXM1 signaling. These results will be beneficial for evaluating nephrotoxicity in chemical risk assessment and for developing new biomarkers to predict nephrotoxicity.

2020-04-01 | GSE144263 | GEO

Comprehensive Expression Analysis of mRNA and microRNA for Investigation of Compensatory Mechanisms in the Rat Kidney after Unilateral Nephrectomy (Male_mRNA)

2020-04-01 | GSE144262 | GEO

Comprehensive Expression Analysis of mRNA and microRNA for Investigation of Compensatory Mechanisms in the Rat Kidney after Unilateral Nephrectomy (Male_miRNA)

2020-04-01 | GSE144261 | GEO

The changes of Chromatin Accessibiliy Triggered by Unilateral Nephrectomy [ATAC-seq]

Project description:Purpose: Experiments were done in mice undergoing unilateral nephrectomy (UNx) and sham nephrectomy. At specific time points (24 hours and 72 hours) after surgery, the earliest first portion of the kidney proximal tubule (PT-S1) and the cortical collecting duct (CCD) were manually micro-dissected and utilized for transcriptomic analysis by single-tubule small sample RNA-Seq and single-tubule ATAC seq. Methods: We carried out ATAC-sequencing in microdissected ealiest portion of proximal tubules (PT-S1) from mice with or without (sham) unilateral nephrectomy (UNx). Each group (Sham or UNx) has 3 biological replicates. Results and conclusion: ATAC-seq peaks in microdissected PT-S1 revealed a predominance of binding site motifs corresponding to PPARα.

2022-08-14 | GSE211022 | GEO

Signaling in Renal Compensatory Hypertrophy Revealed by Multi-Omics_Outer Cortex

Project description:This mouse kidney outer cortex proteomics data set is part of multi-omic approach in a mouse unilateral nephrectomy model to identify signaling processes associated with compensatory hypertrophy of the renal proximal tubule.

2023-06-05 | PXD039697 | Pride

Transcriptome Changes of Microdissected Kidney Tubules triggered by Unilateral Nephrectomy [RNA-seq]

Project description:Purpose: Experiments were done in mice undergoing unilateral nephrectomy (UNx) and sham nephrectomy. At specific time points (24 hours and 72 hours) after surgery, the earliest first portion of the kidney proximal tubule (PT-S1) and the cortical collecting duct (CCD) were manually micro-dissected and utilized for transcriptomic analysis by single-tubule small sample RNA-Seq. Methods: We carried out RNA-sequencing in microdissected ealiest portion of proximal tubules (PT-S1) or cortical collecting duct (CCD) from mice with or without (sham) unilateral nephrectomy (UNx). Each group (Sham or UNx) has 3-5 biological replicates. Results and conclusion: RNA-Seq in microdissected PT-S1 at 24 hours showed that peroxisome proliferator-activated receptor alpha (PPARα) target genes were strongly upregulated. RNA-Seq in microdissected PT-S1 at 72 hours showed that cell cycle related genes were strongly upregulated. RNA-Seq in microdissected CCD at both 24 hours and 72 hours showed that cell cycle related genes were strongly upregulated.

2022-08-14 | GSE211021 | GEO

The transcriptional response to unilateral nephrectomy with and without ischemia-reperfusion injury

Project description:Transcriptome analysis was done after warm renal ischemia-reperfusion injury (IRI) in a rat model. Earlier studies have shown a protective effect of prior unilateral nephrectomy (UNx) against IRI in the remaining, contralateral kidney compared to a non-neprectomized control group. We aimed at identifying the underlying molecular mechanisms. We used the Affymetrix Clariom D array (formerly known as RTA 1.0 st.) Array data was processed in the Affymetrix Console Software.

2017-11-04 | GSE100540 | GEO

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