Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression of cell populations sorted from exocrine cells infected with PNM (M3) or NGN3 and LacZ or REST

ABSTRACT: Gene expression of cell populations sorted from exocrine cells infected with PNM (M3) or NGN3 and LacZ or REST

PROVIDER: PRJNA603389 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10972705.fastq.gz	Fastqsanger.gz
	SRR10972706.fastq.gz	Fastqsanger.gz
	SRR10972707.fastq.gz	Fastqsanger.gz
	SRR10972708.fastq.gz	Fastqsanger.gz
	SRR10972709.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 23

Similar Datasets

Gene expression of cell populations sorted from exocrine cells infected with PNM (M3) or NGN3 and LacZ or REST

Project description:Bulk MARS-seq analysis of gene expression identified genes induced by PNM or NGN3 that are inhibited by REST expression.

2020-03-25 | GSE144337 | GEO

Gene expression of the combined effect of PDX1 and dominant-negative REST (DN-REST) on primary pancreatic exocrine cells

Project description:Bulk MARS-seq analysis of gene expression in exocrine cells infected with Ad-PDX1, Ad-DN-REST or both. DN-REST activated endocrine genes, many of which exhibited a synergistic effect in coexpression with PDX1. PDX1 alone activated exocrine genes.

2020-03-25 | GSE128546 | GEO

Identifying new interactors of NGN3 through IP-MS

Project description:NGN3 is a transcription factor whose transient expression during pancreatic development is vital for the generation of endocrine pancreatic cells, including beta cells. NGN3 stabilisation has been shown to induce exocrine-to-endocrine cell plasticity in the murine pancreas, making it a viable target for therapies aiming to replenish beta cells after immune-mediated destruction in type 1 diabetes patients. Here, we set out to identify new interactors of NGN3 that could play a role in its post-translational regulation. We transfected HEK293A cells with HA-tagged NGN3 and carried out immunoprecipitation of the HA-tag, followed by analysis of co-immunoprecipitated interactors via LC-MS/MS.

2023-03-27 | PXD033691 | Pride

Inducible Ngn3 Embryonic Stem Cells

Project description:Expression of the proendocrine gene neurogenin 3 (Ngn3) is required for the development of pancreatic islets. In order to better characterize the molecular events regulated by Ngn3 during development, we have determined the expression profile of differentiating murine embryonic stem cells (mESCs) uniformly induced to overexpress Ngn3. An ESC line was created that allows for the induction of Ngn3 by adding doxycycline (Dox) to the culture medium. Genome-wide microarray analysis was performed to identify genes regulated by Ngn3 in a variety of both undifferentiated and differentiated conditions. Characterization of pancreatic developmental markers during embryoid body (EB) formation revealed an optimum context for Ngn3 induction. Neuroendocrine genes including neurogenic differentiation 1 (NeuroD1) and single minded 1 (Sim1) were found to be significantly upregulated. Genes regulated by Ngn3 independent of the context were analyzed using systematic gene ontology tools and revealed Notch signaling as the most significantly regulated signaling pathway (p=0.009). This result is consistent with the hypothesis that Ngn3 expression makes the cell competent for Notch signaling to be activated and conversely, more sensitive to Notch signaling inhibition. Indeed, EBs induced to express Ngn3 were significantly more sensitive to gamma-secretase inhibitor-mediated Notch signaling inhibition (p<0.0001). Moreover, we find that Ngn3 induction in differentiating ESCs results in significant increases in insulin, glucagon, and somatostatin transcription. Keywords: Ngn3 induction, embryonic stem cell differentiation

2006-07-01 | GSE3653 | GEO

Gene expression of the combined effect of PDX1 and dominant-negative REST (DN-REST) on primary pancreatic exocrine cells

Project description:Gene expression of the combined effect of PDX1 and dominant-negative REST (DN-REST) on primary pancreatic exocrine cells

| PRJNA528080 | ENA

Gene expression of Cell populations sorted from exocrine cells reprogrammed to beta-like cells

Project description:Bulk MARS-seq analysis of gene expression in reprogrammed exocrine cells exhibit many similarities to native beta cells. Reprogrammed cells are enriched for expression of genes that are direct targets of the transcriptional repressor REST.

2020-03-25 | GSE128545 | GEO

Molecular mechanism of muscarinic acetylcholine receptor M3 interaction with Gq

Project description:Muscarinic acetylcholine receptor M3 (M3) and its downstream effector Gq/11 are critical drug development targets given their involvement in numerous physiological processes and diseases. Although a cryo-electron microscopy study previously defined the structure of the M3-miniGq complex, the lack of information on the intracellular loop 3 (ICL3) of M3 and α-helical domain (AHD) of Gαq has made it difficult to comprehend the molecular mechanism of M3-Gq coupling fully. Here, we present the molecular mechanism underlying the dynamic interactions between the wild-type full-length M3 and heterotrimeric Gq using hydrogen-deuterium exchange mass spectrometry and NanoLuc Binary Technology-based cell systems. This study suggests potential binding interfaces between M3 and Gq in pre-assembled and fully active nucleotide-free complexes. In addition to well-known binding interfaces, we observed the interaction of long ICL3 with Gβγ. Furthermore, M3 ICL3 negatively affected M3-Gq coupling, and the Gαq AHD underwent unique conformational changes during M3-Gq coupling. Therefore, we propose a comprehensive molecular mechanism of M3-Gq coupling by analyzing previously well-defined binding interfaces and neglected regions, such as M3 ICL3 and the Gαq AHD.

2024-05-22 | PXD042562 | Pride

Mycobacterium tuberculosis M3 strain:M3

Project description:Mycobacterium tuberculosis M3 strain:M3 Genome sequencing

| PRJNA183185 | ENA

Vibrio anguillarum M3 strain:M3

Project description:Vibrio anguillarum M3 strain:M3 Genome sequencing

| PRJNA211964 | ENA

Microbacterium sp. M3 strain:M3

Project description:Microbacterium sp. M3 strain:M3 Genome sequencing and assembly

| PRJNA1034264 | ENA