Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from control samples with or without parasite contact at 10 days. Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans
Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from uninfected and infected samples at 3 time points (3, 10 and 20 days). Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans
Project description:In this study we show the transcriptional analysis after shifting the Ustilago maydis from media with ammonium as a nitrogen source to media lacking ammonium. From these, 49 genes were up-regulated and 41 were down-regulated. The functional description and gene ontology terms associated to the diferentially expressed genes revealed that various key pathways were represented, including, secondary metabolism, the metabolism of nitrogen, amino acid, fatty acid and amino sugar, among others, suggesting that the interplay of U. maydis with its N2 fixing bacterial endosymbiont is a flexible process that may be active during the adaptation to the fungus to the different nitrogen sources, and possibly during its pathogenic style of life.
Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from control samples with or without parasite contact at 10 days. Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans RNAs are a mix of Wigglesworthia, Sodalis and glossina. RNAs were extracted from 8 samples including 2 conditions (with 4 replicates per condition).
Project description:Rhizopus microsporus is one of the most common agents of mucormycosis, a severe and emerging infection caused by Mucorales fungi that poses a significant clinical challenge, particularly due to the growing population of immunocompromised individuals. An substantial proportion of clinical isolates harbor bacterial endosymbionts, which regulate key fungal functions, such asexual sporulation. The strict dependence on endosymbionts for spore formation has limited a comprehensive understanding of endosymbiosis in R. microsporus biology. Here, we demonstrate that sporulation in endosymbiont-cured strains of this fungus, which previously harbored Mycetohabitans bacteria, can be induced by light. Interestingly, both light and endosymbionts regulate sporulation through the same regulatory pathway, and a high proportion of the genes upregulated by these signals are known to be involved in asexual sporulation in other fungi, including Mucorales. Light-induced sporulation enabled comparative assays to assess the impact of symbiosis on fungal traits. In addition to previously known effects on fungal fitness, this study reveals that germination is independent of endosymbionts, although their presence accelerates the process. Furthermore, it shows that asexual spores lacking endosymbionts exhibit a reduced virulence in a mouse model of mucormycosis. The discovery of light-induced sporulation in endosymbiont-free strains of R. microsporus paves the way for future comparative studies using genetically identical backgrounds, advancing our understanding of fungal-bacterial symbiosis and its role in fungal biology and human pathogenesis.
Project description:We report the application of Cappable-seq to selectively enrich prokaryotic endosymbiont transcripts from mixed host-symbiont total RNA.
Project description:The transformation of endosymbiotic bacteria into genetically integrated organelles was central to eukaryote evolution. During organellogenesis, control over endosymbiont division, proteome composition, and physiology largely shifted from endosymbiont to nucleus. However, the order and timing of events underpinning this major evolutionary transition are poorly understood. Here, we identified by protein mass spectrometry seven nucleus-encoded proteins that are targeted to the endosymbiont.
Project description:Uric acid stored in the fat body of cockroaches is a nitrogen reservoir mobilized in times of scarcity. The discovery of urease in Blattabacterium cuenoti, the primary endosymbiont of cockroaches, suggests that the endosymbiont may participate in cockroach nitrogen economy. However, bacterial urease may only be one piece in the entire nitrogen recycling process from insect uric acid. Thus, in addition to the uricolytic pathway to urea, there must be glutamine synthetase assimilating the released ammonia by the urease reaction to enable the stored nitrogen to be metabolically usable.
Project description:We sequenced total RNA from Dirofilaria immitis in order to generate the first tissue-specific gene expression profile of a filarial nematode and its Wolbachia endosymbiont.
Project description:Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. A total of 178 novel microRNA were identified from short read transcriptional data, which when combined with known Brugia microRNAs yielded a total of 284 microRNA. Of these, 123 microRNA sequences (43%) are differentially expressed over the mammalian life stages of B. malayi that we examined. Putative targets of these microRNA were identified from inversely expressed target clusters that contain valid seed sequences for the corresponding microRNAs. The largest identified cluster is downregulated in adult females and enriched in zinc finger domains, helicase domains, and DNA binding domains suggesting this microRNA cluster may have regulatory control over a large proportion of adult female specific mRNA genes. MicroRNA-like molecules are identified as produced by the Wolbachia endosymbiont, providing evidence for direct nucleic acid-based interdomain communication between filarial nematodes and their bacterial obligate endosymbiont.
