Project description:Molecular taxonomy and key signaling pathways of apocrine glands of patients with hidradenitis suppurativa were studied by whole transcriptome profiling. Dysregulated genes were detected by comparing lesional and non lesional skin obtained from male and female HS patients using the Agilent array platform.
Project description:Hidradenitis Suppurativa molecular taxonomy and key signaling pathways were studied by whole transcriptome profiling. Dysregulated genes were detected by comparing lesional and non lesional skin obtained from female HS patients and matched healthy controls using the Agilent array platform
Project description:To acquire a better understanding of the molecular pathogenesis of hidradenitis suppurativa (HS), we performed mRNA microarray studies to compare whole blood gene expression of HS patients to that of healthy normal subjects. No significant difference was observed in whole blood mRNA expression between HS patients and healthy normal control. Whole blood samples were collected from patients with hidradenitis (n=16) at baseline and healthy normal subjects (n=10) for RNA extraction and microarray analysis.
Project description:Hidradenitis suppurativa (HS), also termed acne inversa, is a persistent inflammatory dermatological condition affecting approximately 1% of the global population, causing significant morbidity. The etiology of HS is not fully elucidated, but it is known that immune dysfunction plays a critical role. In our research to discern the role of non-coding RNA in HS, we initially conducted a comparative analysis of the most significantly altered long non-coding RNA (lncRNA) and mRNA expressions.
Project description:Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease with a relapsing, remitting course. The disorder is characterized by painful abscesses and nodules in areas with high sweat gland and hair follicle density. The pathogenesis of HS is still incompletely understood. To provide insight into the cellular landscape of HS lesions, we utilized single-cell RNA sequencing (scRNA-seq) technology.
Project description:Hidradenitis suppurativa is a common, debilitating inflammatory skin disease linked to immune dysregulation and abnormalities in follicular structure and function. Few studies have characterized the transcriptomic profile of affected and unaffected skin. We established an RNA-Seq based hidradenitis suppurativa expression disease signature and found it largely concordant with an earlier microarray-based study. We confirmed known aspects of the underlying disease biology including known immune response pathways, differential regulation of antimicrobial peptides, and complement activation. We further characterize the extent of changes in the complement cascade in hidradenitis lesions and highlight a signature that implicates host response to bacteria in disease pathogenesis.
Project description:The aim of this study was to obtain a better understanding of the molecular background of hidradenitis suppurativa (HS). Global transcriptome analysis of lesional and perilesional skin identifies numerous differentially expressed genes that display unique expression pattern in HS. Genes, whose expression is dysregulated in the skin of patients suffering from HS are novel biomarker candidates and potential therapeutic targets for HS.
Project description:This study investigated the underlying inflammatory pathways and cell types in hidradenitis suppurativa using transcriptomic approaches with RNA sequencing of lesional and non-lesional skin biopsies from hidradenitis suppurativa patients.
Project description:To acquire a better understanding of the molecular pathogenesis of hidradenitis suppurativa (HS), we performed mRNA microarray studies to compare whole blood gene expression of HS patients to that of healthy normal subjects. No significant difference was observed in whole blood mRNA expression between HS patients and healthy normal control.