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Lates calcarifer

ABSTRACT: Asian seabass Transcriptome

PROVIDER: PRJNA605304 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11075859.fastq.gz	Fastqsanger.gz
	SRR11075860.fastq.gz	Fastqsanger.gz
	SRR11075861.fastq.gz	Fastqsanger.gz
	SRR11075862.fastq.gz	Fastqsanger.gz
	SRR11075863.fastq.gz	Fastqsanger.gz

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Lates calcarifer

Project description:Asian seabass transcriptome

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Microarray analysis of the Asian seabass (Lates calcarifer) brains

Project description:In this study, we attempt to characterize the transcriptomic profile of the Asian seabass brains collected from the male and female sexes. The objective is to identify genes that show sexually dimorphic expression in the brain of this species. For this experiment, Asian seabass were collected from the Marine Aquaculture Center of the Agri-Food & Veterinary Authority of Singapore. There were no treatments carried out in this experiment. Four brains from adult male seabass (5 years old) with M3-type testis and four brains from adult female seabass (5 years old) with F3-type ovaries were used in this experiment. (Gonads were examined by histology and classified according to sexual maturation status as described by Guiguen and colleagues (Guiguen et al. Environmental Biology of Fishes, 1994)).

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Microarray analysis of the Asian seabass (Lates calcarifer) gonads

Project description:In this study, we attempt to characterize the transcriptomic profile of the Asian seabass gonads at various developmental stages. The protandric Asian seabass or barramundi (Lates calcarifer) typically matures as a male at approximately 2–4 years of age and then changes sex to a female in later years. For this experiment, Asian seabass of several ages were collected from the Marine Aquaculture Center of the Agri-Food & Veterinary Authority of Singapore and from farms around Singapore. There were no treatments carried out in this experiment. The gonads were examined by histology and classified according to sexual maturation status as described by Guiguen and colleagues (Guiguen et al. Environmental Biology of Fishes, 1994). Altogether, we analyzed 22 gonadal samples that could be classified into six different types of gonads.

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Lates calcarifer

Project description:Asian seabass transcriptome (part 2)

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Microarray analysis of the Asian seabass (Lates calcarifer) gonads

Project description:In this study, we attempt to characterize the transcriptomic profile of the Asian seabass gonads at various developmental stages. The protandric Asian seabass or barramundi (Lates calcarifer) typically matures as a male at approximately 2M-bM-^@M-^S4 years of age and then changes sex to a female in later years. For this experiment, Asian seabass of several ages were collected from the Marine Aquaculture Center of the Agri-Food & Veterinary Authority of Singapore and from farms around Singapore. There were no treatments carried out in this experiment. The gonads were examined by histology and classified according to sexual maturation status as described by Guiguen and colleagues (Guiguen et al. Environmental Biology of Fishes, 1994). Altogether, we analyzed 22 gonadal samples that could be classified into six different types of gonads. Total 22 samples: Adult Ovaries (F3-stage; 5 years old fish) : 4 Adult Testes (M3-stage; 5 years old fish) : 4 Early Testes (M3-stage; 8-9 months old fish) : 3 Early Transforming Gonads (>2 years old fish) : 3 Late Transforming Gonads (>2 years old fish) : 4 Undifferentiated Gonads (4.5 months old fish) : 4

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Project description:Asian seabass (Lates calcarifer) Brain RNA-seq