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FGFR3 is a positive regulator of osteoblast expansion and differentiation during zebrafish skull vault development

ABSTRACT: FGFR3 is a positive regulator of osteoblast expansion and differentiation during zebrafish skull vault development

PROVIDER: PRJNA605419 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11040628_1.fastq.gz	Fastqsanger.gz
	SRR11040628_2.fastq.gz	Fastqsanger.gz
	SRR11040629_1.fastq.gz	Fastqsanger.gz
	SRR11040629_2.fastq.gz	Fastqsanger.gz
	SRR11040630_1.fastq.gz	Fastqsanger.gz

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FGFR3 is a positive regulator of osteoblast expansion and differentiation during zebrafish skull vault development

Project description:In order to study FGFR3’s role in cranial vault development, we generated the first fgfr3 loss-of-function zebrafish (fgfr3lof1/lof1). The mutant fish exhibited major changes in the craniofacial skeleton, with a lack of sutures, abnormal frontal and parietal bones, and the presence of ectopic bones. In order to define cellular and molecular processes responsible for this phenotype, we performed single-cell RNA sequencing of cranial vault cells from fgfr3lof1/lof1 and fgfr3+/+ zebrafish. Our data allow us to define transcriptomic profile of each osteogenic subpopulation involved in cranial vault formation and we revealed a defect in osteoblast differentiation associated with changes in the extracellular matrix. These findings demonstrate that fgfr3 is a positive regulator of osteogenesis and we conclude that changes in the extracellular matrix within growing bone impair cell-cell communication, mineralization, and new osteoblast recruitment.

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Microarray of Human astrocytes transduced with lentivirus expressing FGFR3-TACC3 treated with vehicle, FGFR3-TACC3 treated with the FGFR inhibitor PD173074 for 12h, FGFR3-TACC3-K508M treated with vehicle or empty vector treated with vehicle

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