Epigenome analysis of placental DNA methylation and added sugar intake during pregnancy
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ABSTRACT:
PROVIDER: PRJNA605548 | ENA |
REPOSITORIES: ENA
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Project description:Genome wide DNA methylation profiling of placenta at birth. A 1 cm3 sample of the placenta was collected (using scissor, midway between the umbilical cord and the outer edge of the placenta) and genomic DNA was extracted. DNA integrity was assessed by agarose gel electrophoresis with ethidium bromide staining and PicoGreen (Life Technologies, Carlsbad, Calif.). Starting with 500 ng of genomic DNA, bisulfite conversion and methylation profiling was performed according to the manufacturer’s instructions for Illumina Human Methylation 450K BeadChips. All assays were performed at the Roswell Park Cancer Institute Genomics Shared Resource. After background correction and normalization to internal controls, Genome Studio software was used to calculate the fractional methylation (AVG_Beta) at each CpG (N= 453,144 CpG sites). Samples includes XX placenta.
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2012-07-12 | E-GEOD-39290 | biostudies-arrayexpress
Project description:To explore the influence of maternal choline intake on placental gene expression, we employed whole genome microarray expression profiling to identify genes that were differentially expressed in placental tissues obtained from women consuming two different doses (480 vs. 930 mg/d) of choline throughout the third trimester of pregnancy. Healthy third trimester (gestational week 26-29) pregnant women were randomized to a 12-week choline controlled feeding study. The participants consumed either 480 (n=6) or 930 (n=6) mg choline/d. Full thickness placental samples were collected at delivery to extract RNA and perform the arrays.
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Project description:Maternal obesity is becoming a major health consideration for successful pregnancy outcomes. There is growing proof that maternal obesity has a negative influence on placental development and function, thereby adversely influencing offspring programming and health outcomes. However, the molecular mechanisms underlying these processes are so far poorly understood. We set out to analyse term placenta whole transcriptome in obese (n=5) and normoweight women (n=5), using Affymetrix microarray platform compromising of 50,000 probe sets. Our analysis shows that the placental transcriptome differs between normoweight and obese women. Different processes and pathways among placenta from obese women were dysregulated, including inflammation and immune responses, lipid metabolism, cell death and survival and cancer pathways, vasculogenesis and angiogenesis, and glucocorticoid receptor signaling pathway. Together, this global gene expression profiling approach demonstrates and confirms that maternal obesity creates a unique in utero environment that impairs placental transcriptome.
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Project description:We conducted a preliminary investigation to determine whether ethanol-induced alterations in placental gene expression may have some utility as a diagnostic indicator of maternal drinking during pregnancy as well as a prognostic indicator of risk for adverse neurobehavioral outcomes in affected offspring.
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