Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome profiling of WM3248 melanoma cells following PGC1-alpha knockdown

ABSTRACT: Transcriptome profiling of WM3248 melanoma cells following PGC1-alpha knockdown

PROVIDER: PRJNA606044 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11067676.fastq.gz	Fastqsanger.gz
	SRR11067677.fastq.gz	Fastqsanger.gz
	SRR11067678.fastq.gz	Fastqsanger.gz
	SRR11067679.fastq.gz	Fastqsanger.gz
	SRR11067680.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Transcriptome profiling of WM3248 melanoma cells following PGC1-alpha knockdown

Project description:PGC1-alpha is a transcriptional coactivator that controls diverse cellular processes, including metabolism. PGC1-alpha expression is increased in invasive primary melanoma. To understand the role of PGC1-alpha in the invasive growth of primary melanoma, we generated transcriptional (RNA-Seq) profiles of WM3248 primary melanoma cells that were transfected with either negative control or PGC1-alpha siRNA.

2020-02-12 | GSE145101 | GEO

RNA-seq analysis of SKMEL28 melanoma cells following DIRC3 and IGFBP5 ASO knockdown

Project description:RNA-seq analysis of SKMEL28 melanoma cells following DIRC3 and IGFBP5 ASO knockdown

| PRJNA531445 | ENA

Gene expression profiling following the overexpression or the knockdown of Ambra1 in A375 melanoma cell lines

Project description:Gene expression profiling following the overexpression or the knockdown of Ambra1 in A375 melanoma cell lines

| PRJNA732077 | ENA

Transcriptional profiling following knockdown of RNA binding proteins

Project description:Transcriptional profiling following knockdown of RNA binding proteins

| PRJNA642291 | ENA

Transcriptome profiling following knockdown of NAA50 in Arabidopsis

Project description:Transcriptome profiling following knockdown of NAA50 in Arabidopsis

| PRJNA607603 | ENA

Gene expression profiling following the overexpression or the knockdown of Ambra1 in A375 melanoma cell lines

Project description:Melanoma is the most deadly type of skin cancers and, there is a continuous need to develop early biomarkers as well as treatments to improve the survivability of patients. Ambra1 is an essential regulator in autophagy and it has been recently shown to have a role in cell proliferation. We used microarrays to analyse the transcriptomic changesi n A375 melanoma cell lines following Ambra1 differential expression

2021-05-23 | GSE175361 | GEO

Gene expression profiling of mouse macrophages following IRF2 knockdown

Project description:Gene expression profiling of mouse macrophages following IRF2 knockdown

| PRJNA418340 | ENA

Expression profiling by high-throughput RNA-sequencing following PGC1β and ERRα knockdown in HCT116 cells

Project description:The goal of this experiment was to determine differential gene regulation of PGC1 or ERR compared to the control.

2020-04-02 | GSE147905 | GEO

Naa10p Inhibits Beige Adipocyte-mediated Thermogenesis through N-alpha-acetylation of Pgc1-alpha

Project description:Naa10p Inhibits Beige Adipocyte-mediated Thermogenesis through N-alpha-acetylation of Pgc1-alpha

| PRJNA552345 | ENA

PDGFR-alpha inhibits melanoma growth via CXCL10/IP-10 and miR-503. A multi-omics approach.

Project description:Cutaneous melanoma is the most aggressive skin cancer showing high mortality at advanced clinical stages. Platelet-Derived Growth Factor Receptor alpha (PDGFR-alpha) is known to strongly inhibit melanoma and endothelial cells proliferation, in vitro as well in vivo. PDGFR-alpha expression has been found to be reduced in metastatic human melanoma-biopsies, as compared to benign nevi-biopsies, thus implying a negative selection of PDGFR-alpha expressing cells, in melanoma. In the present study PDGFR-alpha was transiently overexpressed in endothelial (HUVEC) and melanoma (SK-Mel-28) human cells; a strong anti-proliferation effect was observed, along with profound effects on mRNA and miRNA expression. In detail, gene-expression profiling showed that PDGFR-alpha over-expression affects the expression of 82 transcripts in HUVEC (41 up-, 41 down-regulated), and 52 Transcripts in SK-Mel-28 (43 up-, 9 down-regulated). Finally, a miRNA profiling showed that 14 miRs are up-regulated and 39 are down-regulated in PDGFR-alpha overexpressing cells. Accurate validation with alternative techniques demonstrated that CXCL10 is one of the most significantly up-regulated at both gene- and protein level, in combination with a strong down-regulation of miR-503 in both HUVEC and SK-Mel-28 overexpressing PDGFR-alpha. We then demonstrate that CXCL10 is a validated miR-503 target, and that the anti-proliferation effect of PDGFR-alpha is reverted by specific CXCL-10 neutralization. In conclusion, PDGFR-alpha overexpression strongly inhibits endothelial- and melanoma- proliferation in a CXCL-10 dependent way, by significantly down-regulating miR-503 expression. This data set contains the results of the mRNA analysis.

2016-11-14 | E-MTAB-4261 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data