Project description:This is a prevalence study evaluating lower urinary tract, prolapse, bowel, and sexual symptoms in women with a colorectal disorder who are planning to undergo surgery.
The purpose of this study is to identify the number of women who complain of lower urinary tract and bowel problems, including frequency, urgency, urinary incontinence, fecal incontinence, pain with intercourse, and other sexual problems prior to undergoing surgical management for a colorectal disorder.
Project description:RIVUR Trial participants had Agilent 1M probe and or Nimblegen 2.1M probe aCGH performed on genomic DNA. The study was designed to discover DNA copy number variations in genes critical in kidney/urinary tract development and urinary tract infection susceptibility. Reference DNA used is a single male sample
Project description:<p><strong>BACKGROUND:</strong> Protein undernutrition is prevalent mainly among elderly people, and it could develop diseases such as sarcopenia. Urinary metabolites may become biomarkers reflecting protein undernutrition, but this has been insufficiently investigated.</p><p><strong>OBJECTIVE:</strong> The purpose of this study was to identify novel urinary metabolites as biomarker candidates responsive to protein undernutrition.</p><p><strong>METHODS:</strong> Adult male Wistar rats were fed an AIN-93 M diet [14% casein, control (CT) diet] or an AIN-93 M-based isocaloric diet [5% casein, low protein (LP) diet] for 4 weeks. 1H nuclear magnetic resonance metabolomic analysis was performed on urine samples weekly and on plasma and liver samples at week 4 to identify metabolites that respond to protein undernutrition. Liver samples were also subjected to mRNA microarray and quantitative PCR analyses at week 4 to investigate alterations of the gene expressions responsive to protein undernutrition.</p><p><strong>RESULTS:</strong> Urinary taurine levels were significantly lower in the LP group than in the CT group (79.7% lower) at week 1, and remained constant until week 4. Hepatic taurine level and gene expression level of cysteine dioxygenase type 1, the rate-limiting enzyme for taurine biosynthesis, were also significantly lower in the LP group than in the CT group (81.1% and 32.5% lower, respectively). Urinary trimethylamine N-oxide (TMAO) levels were significantly higher in the LP group than in the CT group (49.7% higher) at week 2, and remained constant until week 4. Hepatic TMAO level was also higher in the LP group than in the CT group (81.2% higher). Hepatic gene expression levels of flavin-containing monooxygenase 1 and 5, the enzymes for TMAO biosynthesis, were also significantly higher in the LP group than in the CT group (61.6% and 311.8% higher, respectively).</p><p><strong>CONCLUSIONS:</strong> Urinary taurine and TMAO levels substantially respond to LP diet ingestion, and may act as non-invasive biomarker candidates to detect protein undernutrition.</p>
Project description:The autonomic nervous system is derived from the neural crest and supplies motor innervation to the smooth muscle of visceral organs, including the lower urinary tract (bladder and urethra, LUT). In rodents, autonomic innervation of the LUT is supplied by the major pelvic ganglia (PG) that lie near the neck of the bladder and proximal urethra. Compared to other autonomic ganglia, the PG are unique in that they harbor both sympathetic and parasympathetic neurons. The coordinated activity of PG neurons is critical for normal functioning of the LUT – however, surprisingly little is known about how PG neuronal diversity is established or what molecular factors control PG development. In this study we conducted transcriptome profiling of Sox10-H2BVenus+ sacral neural crest (NC) progenitors to discover candidate genes involved in PG neurogenesis.
Project description:Exercise could stimulate the release of exosomes into the circulation, transferring signals between the cells. Exosomes are also found in urine, which is an emerging biomarker of several diseases. However, the characteristics of urinary exosomes and their contents after exercise remain poorly understood. We used microarrays to identify the alteration of gene expression in urinary exosomes after exercise bout.