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The effects of low-level Er:YAG laser irradiation on proliferation and calcification of primary osteoblast-like cells isolated from rat calvaria

ABSTRACT: The effects of low-level Er:YAG laser irradiation on proliferation and calcification of primary osteoblast-like cells isolated from rat calvaria

PROVIDER: PRJNA609280 | ENA |

REPOSITORIES: ENA

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The effects of low-level Er:YAG laser irradiation on proliferation and calcification of primary osteoblast-like cells isolated from rat calvaria

Project description:A number of reports showed that photo-bio-modulation by various lasers has favorable biological effects on cells. However, the effects of low-level Er:YAG laser irradiation on osteoblasts remain unclear. The purpose of this study was to evaluate the effects on proliferation and osteogenic differentiation of primary osteoblast-like cells by low-level Er:YAG laser irradiation. Osteoblast-like cells isolated from the calvariae of 3–5-day-old Wistar rats were irradiated by Er:YAG laser at energy fluences of 2.2, 3.3, or 4.3 J/cm2, respectively. After irradiation, cell surface temperature was measured and cell proliferation was evaluated by flow cytometry. Calcification was evaluated by measuring the Alizarin red S staining area after 7-day-culture with osteoinductive medium. Gene expressions in non-irradiated and laser-irradiated cells were evaluated by qPCR at 3, 6, and 12 hours after irradiation. Microarray analysis was performed to comprehensively evaluate gene expressions of non-irradiated cells and irradiated cells at 3.3 J/cm2 at 6 hours after irradiation. No pronounced increase of cell surface temperature was induced by low-level Er:YAG laser irradiation, and the Er:YAG laser irradiation did not affect osteoblast-like cell proliferation. Osteoblast-like cell calcification was significantly increased 7 days after Er:YAG laser irradiation at 3.3 J/cm2. Bglap expression was significantly increased in cells irradiated at 3.3 J/cm2 at 6 hours post-irradiation. Microarray analysis showed that irradiation at 3.3 J/cm2 caused up-regulation of inflammation-related genes and down-regulation of Wisp2. Gene set enrichment analysis also clarified enrichment of inflammation-related gene sets and Notch signaling pathway. In conclusion, Low-level Er:YAG laser irradiation enhanced calcification of primary osteoblast-like cells via enhanced Bglap expression and enriched Notch signaling pathway.

2022-02-28 | GSE146098 | GEO

Effects of Low-Level Er:YAG Laser Irradiation on Proliferation and Calcification of Primary Osteoblast-Like Cells Isolated From Rat Calvaria.

Project description:Several reports have shown that the photo-bio-modulation of cells by various lasers has favorable biological effects. However, the effects of low-level Er:YAG laser irradiation on osteoblasts remain unclear. The purpose of this study was to evaluate the effects of low-level Er:YAG laser irradiation on proliferation and osteogenic differentiation of primary osteoblast-like cells isolated from the calvariae of 3-5-day-old Wistar rats. Cells were irradiated by Er:YAG laser at energy fluences of 2.2, 3.3, and 4.3 J/cm2, respectively. After irradiation, cell surface temperatures were measured and cell proliferation was evaluated by flow cytometry and CCK-8. Calcification was evaluated by measuring areas of Alizarin red S staining after 7, 14, and 21 days culture in osteoinductive medium. Gene expression in non-irradiated and laser-irradiated cells was evaluated by qPCR at 3, 6, and 12 h, as well as 1, 3, 7, and 14 days after irradiation. Microarray analysis was performed to comprehensively evaluate the gene expression of non-irradiated and irradiated cells at 3.3 J/cm2 at 6 h after irradiation. No pronounced increase of cell surface temperature was induced by irradiation. Irradiation did not affect osteoblast-like cell proliferation. Osteoblast-like cell calcification was significantly increased 7 days after Er:YAG laser irradiation at 3.3 J/cm2. Bglap expression was significantly increased in cells irradiated at 3.3 J/cm2 6 h post-irradiation. Microarray analysis showed that irradiation at 3.3 J/cm2 caused an upregulation of inflammation-related genes and downregulation of Wisp2. Gene set enrichment analysis also clarified enrichment of inflammation-related and Notch signaling gene sets. In conclusion, low-level Er:YAG laser irradiation at 3.3 J/cm2 enhanced calcification of primary osteoblast-like cells via enhanced Bglap expression and enriched Notch signaling.

| S-EPMC7324552 | biostudies-literature

Er:YAG laser irradiation decreases Sost expressions in bone and osteogenic cell (24h after surgery)

Project description:The purpose of this study was to evaluate comprehensive and sequential gene expressions in Er;YAG laser ablated bone, bur-drilled bone, and non-treated control bone to clarify the biological responses for bone healing.

2020-03-03 | GSE146181 | GEO

Er:YAG laser irradiation decreases Sost expressions in bone and osteogenic cell (72h after surgery)

2020-03-03 | GSE146182 | GEO

Er:YAG laser irradiation decreases Sost expressions in bone and osteogenic cell (6h after surgery)

2020-03-03 | GSE146180 | GEO

Effect of low-level Er:YAG laser irradiation on proliferation and gene expression changes of primary gingival fibroblasts isolated from mouse maxilla.

Project description:This study aimed to explore the effects of low-level Er:YAG laser irradiation on the proliferation of gingival fibroblasts without the photothermal effect.

2024-03-17 | GSE227558 | GEO

Er:YAG laser irradiation decreases Sost expressions in bone and osteogenic cell

Project description:Er:YAG laser irradiation decreases Sost expressions in bone and osteogenic cell

| PRJNA609661 | ENA

Transcriptional profiling by microarray of mRNAs of osteoblastic cells from calvaria and bone marrow of ovariectomized rats.

Project description:The mRNA oligo microarrays from Agilent were used to evaluate behavioral differences in osteoblast-like cells from calvaria and bone marrow of rats induced to osteoporosis by ovariectomy. Microarray data were normalized using quantile and after statistical analysis by unpaired t-test with p-value ≤ 0.005 and subsequent fold change ≥ 2.0, 5.447 differentially expressed mRNAs were detected in calvaria cells and 4.399 mRNAs in bone marrow cells.

2015-05-25 | E-MTAB-3564 | biostudies-arrayexpress

Transcriptional profiling by microarray of microRNAs of osteoblastic cells from calvaria and bone marrow of ovariectomized rats.

Project description:The microRNA oligo microarrays from Agilent were used to evaluate behavioral differences in osteoblast-like cells from calvaria and bone marrow of rats induced to osteoporosis by ovariectomy. The miRNAs data were normalized using the quantile and after statistical analysis by moderate t-test with p-value ≤ 0.05 and subsequent fold change ≥ 1.5, 84 miRNAs differentially expressed in calvaria cells were detected and 55 miRNAs in bone marrow cells.

2015-05-25 | E-MTAB-3563 | biostudies-arrayexpress

The global gene expression profile of gain of function miR34c in osteoblast

Project description:To examine the unbiased global gene expression of gain of miR34c in committed osteoblast in mouse we have performed expression microarray. This approach will allow us to define the novel targets that are negatively regulated by over-expression of miR34c in osteoblast. Total RNA was isolated from primary calvaria from WT and GOF miR34c mouse (n=3) at the age of P3. WT primary calvarial samples were served as control.

2012-05-02 | E-GEOD-36780 | biostudies-arrayexpress

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