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Synergic effects of temperature and irradiance on the physiology of the marine Synechococcus strain WH7803

ABSTRACT: Synergic effects of temperature and irradiance on the physiology of the marine Synechococcus strain WH7803

PROVIDER: PRJNA609760 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11215800_1.fastq.gz	Fastqsanger.gz
	SRR11215800_2.fastq.gz	Fastqsanger.gz
	SRR11215900_1.fastq.gz	Fastqsanger.gz
	SRR11215900_2.fastq.gz	Fastqsanger.gz
	SRR11216000_1.fastq.gz	Fastqsanger.gz

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Synergic effects of temperature and irradiance on the physiology of the marine Synechococcus strain WH7803

Project description:Understanding how microorganisms adjust their metabolism to maintain their ability to survive to short-term environmental variations constitutes one of the major current challenges in microbial ecology. Here WH7803, the best physiologically characterized marine Synechococcus strain was submitted to modulated light/dark cycles or acclimated to continuous high-light (HL) or low-light (LL), then shifted to various stress conditions, including low (LT) or high temperature (HT), HL and ultraviolet (UV) radiations. Physiological responses were analyzed by measuring time courses of photosystem II (PSII) quantum yield, PSII repair rate, pigment and lipid content as well as whole transcriptomes. This notably revealed that cells previously acclimated to HL seem to be better prepared than LL-acclimated cells to sustain an additional light or UV stress, but not a LT stress. Indeed, LT seems to induce a synergic effect with the HL treatment, as previously observed with oxidative stress. While all tested shift conditions induced the downregulation of many photosynthetic genes, notably those encoding PSI, cytochrome b6/f and phycobilisomes, UV stress proved to be more deleterious for PSII than the other treatments, and full recovery of damaged PSII from UV stress seemed to involve the neo-synthesis of a fairly large number of PSII subunits and not just the reassembly of pre-existing subunits after D1 replacement. In contrast, genes involved in glycogen degradation and carotenoid biosynthesis pathways were more particularly upregulated in response to LT. Altogether, these experiments allowed us to identify responses common to all stresses and those more specific to a given stress, thus highlighting genes potentially involved in niche acclimation of this key component of marine ecosystems. Our data also highlighted important specificities of the stress responses compared to model freshwater cyanobacteria.

2020-06-17 | GSE146246 | GEO

Expression of intergenic regions of Synechococcus WH 7803 under environmentally relevant stresses

2012-03-31 | GSE28263 | GEO

Expression of intergenic regions of Synechococcus WH 7803 under environmentally relevant stresses

Project description:We constructed a tiling microarray, covering nearly all of the intergenic regions larger than 50 bp on both strands of the genome of the marine picocyanobacterium Synechococcus WH7803. We analyzed transcript levels from cultures grown under ecologically relevant stress conditions. The investigated stress conditions were cold stress, high light stress, phosphate depletion and iron depletion. We identified several previously unknown small RNAs, partially differentially expressed. The detected RNAs provide a starting point for further investigations on the acclimatisation to different stresses for Synechococcus WH7803. For every applied growth condition the cultures were grown in triplicates as were the respective controls. Respective controls were treated the same as the stressed bacterial cultures in terms of centrifugation and / or dilution. Bacteria were harvested by rapid filtering and directly freezed by liquid nitrogen.

2012-03-30 | E-GEOD-28263 | biostudies-arrayexpress

Low temperature adaptation in marine Synechococcus

Project description:Low temperature acclimation response was compared between two strains of Synechococcus, WH8102 and BL107. Differential protein expression was compared when the strains were grown at 18°C and 22°C.

2015-10-27 | PXD002914 | Pride

Transcription profiling of cyanophage S-PM2 as it infects Synechococcus WH7803 over a 9 hr time series

Project description:To monitor the expression of cyanophage S-PM2 genes as it infects Synechococcus WH7803 over a 9 hr time course. Samples were take from 3 independent cultures. A reference design was used with dye swaps (technical replicates) for each time point.

2009-10-01 | E-MAXD-26 | biostudies-arrayexpress

Transcriptiome dynamics of cyanopodovirus S-SBP1 and its host Synechococcus sp. WH7803

Project description:Whole-genome expression dynamics of cyanopodovirus P-SSP7 and its host Prochlorococcus strain MED4 have been reported. To investigate whether cyanopodoviruses infecting Prochlorococcus and Synechococcus have similar transcription strategy and host response to phage infection, genomic and transcriptomic analyses were conducted on cyanopodovirus S-SBP1 that infects Synechococcus strain WH7803. S-SBP1 has a latent period of 8 h and a burst size of 30 progeny phages per cell. S-SBP1 was most similar to cyanopodovirus S-RIP2 that also infects Synechococcus WH7803, in terms of whole genome phylogenetic relationship and average nucleotide identity (ANI). Three hypervariable genomic islands were found when comparing the genomes of S-SBP1 and S-RIP2, and single nucleotide variants (SNV) were observed on three genes of S-SBP1, which are located within the island regions. Based on RNA-seq analysis, genes of S-SBP1 were clustered into three temporal express classes, with gene content within each class similar to that of P-SSP7. Thirty-two host genes were upregulated during phage infection, including those involved in carbon metabolism, ribosome component and stress response. These upregulated genes were also similar to those of Prochlorococcus MED4 in response to infection by P-SSP7. Our study demonstrates a programmed temporal expression pattern of cyanopodoviruses and hosts during infection.

2020-12-07 | GSE150732 | GEO

Photophysiological and Genetic Factors Underlying Fast Photosynthetic Growth of Unicellular Cyanobacterium Synechococcus sp. PCC 7002

Project description:Application of genome-scale 'omics approaches to dissect subcellular pathways and regulatory networks governing the fast-growing response of Synechococcus sp. PCC 7002 response to variable irradience levels. We employed controlled cultivation and next-generation sequencing technology to identify transcriptional responses of euryhaline unicellular cyanobacterium Synechococcus sp. PCC 7002 grown under steady state conditions at six irradiance levels ranging from 33 to 760 µmol photons m-2 sec-1.

2015-09-04 | E-GEOD-72691 | biostudies-arrayexpress

Marine Synechococcus sp. strain WH7803 shows specific adaptative responses to assimilate nanomolar concentrations of nitrate

Project description:Marine Synechococcus, together with Prochlorococcus, contribute to a significant proportion of the primary production on Earth. The spatial distribution of these two groups of marine picocyanobacteria depends on different factors such as nutrients availability or temperature. Some Synechococcus ecotypes thrive in mesotrophic and moderately oligotrophic waters, where they exploit both oxidized and reduced forms of nitrogen. Here, we present a comprehensive study, which includes transcriptomic and proteomic analyses of the response of Synechococcus sp. strain WH7803 to nanomolar concentrations of nitrate, compared to ammonium or nitrogen starvation. We found that Synechococcus has a specific response to nanomolar nitrate concentration that differs to the response showed under nitrogen starvation or the presence of standard concentrations of either ammonium or nitrate. This fact suggests that the particular response to the uptake of nanomolar concentration of nitrate could be an evolutionary advantage for marine Synechococcus against Prochlorococcus in the natural field.

2022-07-11 | PXD031142 | Pride

Exoproteomes of marine cyanobacteria grown under different regimes to detect their pili production; analysed by LC-MS/MS

Project description:Exoproteomes generated from Synechococcus sp. WH7803 and Prochlorococcus sp. MIT9313 cultures grown under different nutrient, light and temperature conditions. The aim was to see how the production of the pili were affected. Exoproteomes of marine Synechococcus under different nutrient limitations analysed by LC-MS/MS

2021-02-24 | PXD018394 | Pride

Exoproteomes of marine cyanobacteria grown under different regimes to detect their pili production; analysed by LC-MS/MS

2021-02-24 | PXD018524 | Pride