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Cu contaminated soil metagenome

ABSTRACT: Cu contaminated soil metagenome Targeted loci environmental

PROVIDER: PRJNA609894 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11233357_1.fastq.gz	Fastqsanger.gz
	SRR11233357_2.fastq.gz	Fastqsanger.gz
	SRR11233358_1.fastq.gz	Fastqsanger.gz
	SRR11233358_2.fastq.gz	Fastqsanger.gz
	SRR11233359_1.fastq.gz	Fastqsanger.gz

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Effect of Cu-Nanoparticles versus Cu-salt in Enchytraeus albidus (Oligochaeta): differential gene expression through microarray analysis

Project description:Despite the increased utilization of nanoparticles, the behavior and effect in the environment is largely unknown and few resources are available for health and environmental effect studies. Enchytraeids are extensively used in studies of soil ecotoxicology and recently, a cDNA microarray for Enchytraeus albidus was developed, allowing also toxicogenomic studies in this species. These organisms are ecologically relevant small worms that indirectly contribute to the regulation and degradation of organic matter. In this study we compared the gene expression profiles of E. albidus when exposed to copper-salt (CuCl2) and copper nanoparticles (Cu-NP) spiked soil. The worms were exposed for 48 hours in soil to a range of concentrations. Microarray hybridizations revealed different response patterns between copper-salt and copper nanoparticles exposed organisms, these differences are mainly related with transcripts involved in the energy metabolism of the organisms. Despite unknown gene function in the data-set there are indications that Cu-salt and Cu-NP exposure induced specific gene level responses.

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Project description:soil metagenome Targeted loci environmental

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