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YerA41, a Yersinia ruckeri bacteriophage: determination of non-sequencable bacteriophage genome and investigation of bacterial response to infection.

ABSTRACT: YerA41, a Yersinia ruckeri bacteriophage: determination of non-sequencable bacteriophage genome and investigation of bacterial response to infection.

PROVIDER: PRJNA610075 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11232747_1.fastq.gz	Fastqsanger.gz
	SRR11232747_2.fastq.gz	Fastqsanger.gz
	SRR11232748_1.fastq.gz	Fastqsanger.gz
	SRR11232748_2.fastq.gz	Fastqsanger.gz
	SRR11232749_1.fastq.gz	Fastqsanger.gz

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YerA41, a Yersinia ruckeri bacteriophage: determination of non-sequencable bacteriophage genome and investigation of bacterial response to infection.

Project description:YerA41 is a myoviridae bacteriophage that was originally isolated due its ability to infect Yersinia ruckeri bacteria, the causative agent of enteric redmouth disease of salmonid fish. Several attempts to determine its genomic DNA sequence using traditional and next generation sequencing technologies failed, indicating that the phage genome is modified such way that it is an unsuitable template for PCR amplification and sequencing. To determine the YerA41 genome sequence we isolated RNA from phage-infected Y. ruckeri cells at different time points post-infection, and sequenced it. The host-genome specific reads were substracted and de novo assembly was performed on the unaligned reads.

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