Project description:16s RNA gene sequencing data from seawater, bed sediment and steel corrosion samples from Shoreham Harbour, UK, collected to allow bacterial species comparisons between microbially influenced corrosion, the surrounding seawater, and the sea bed sediment at the seafloor and 50cm depth below seafloor.
Project description:The Crown-of-Thorns starfish (COTS), Acanthaster planci, is a highly fecund predator of reef-building corals distributed throughout the Indo-Pacific. COTS population outbreaks cause substantial loss of coral cover, diminishing the integrity and resilience of the reef ecosystems thus increasing their susceptibility to climate change. We sequenced genomes of A. planci from the Great Barrier Reef, Australia (GBR) and Okinawa, Japan (OKI) to guide identification of species-specific peptide communication with potential applications in mitigation strategies. The genome-encoded proteins excreted and secreted into the surrounding seawater by COTS forming aggregations and by those escaping the predatory giant triton snail, Charonia tritonis, were identified LC-MS/MS.
Project description:On August 22 – 25, 2017, 3 colonies of Montipora capitata with a diameter of ~ 24 cm were collected from the inner lagoon surrounding the Hawaii Institute of Marine Biology (HIMB). The corals were acclimated in outdoor flow-through tanks at HIMB that were supplied with ambient seawater and covered with shade-cloth to mimic PAR levels on the reef. Each colony was split in half with a hammer and chisel to produce two colonies of identical genotype, so that each genotype would experience both ambient and increased temperatures. On August 28 - 29, the corals were equally divided among the experimental tanks, at ambient (n=3) or increased (n=3) temperature. Throughout the temperature treatment, corals were randomly rotated among tanks to minimize potential tank effects. On September 1, the temperature in the increased temperature tanks was turned up 2°C per day, 1°C at 0900 and 1°C at 1400, for four days, reaching an average temperature of 30 °C. The ambient tanks averaged 25 °C. On September 26, the heaters were turned off and the temperature returned to ambient levels by Sept. 29.
Project description:Gene expression profiling of corals exposed to control (ambient seawater) or 50 ppb copper for 24 hours Two-condition experiment, Control vs 50ppb. Biological replicates: 5 genotypes paired in control vs 50ppb, each genotype collected from different location on reef. One replicate per array.
Project description:Soft corals (Cnidaria, Anthozoa, Octocorallia) are a diverse group of marine invertebrates that inhabit various marine environments in tropical and subtropical areas. Several species are recognized as prolific sources of compounds with a wide array of biological activities. Recent advances in analytical techniques, supported by robust statistical analyses, have allowed the analysis and characterization of the metabolome present in a single living organism. In this study, a liquid chromatography-high resolution mass spectrometry metabolomic approach was applied to analyze the metabolite composition of 28 soft corals present in the Caribbean coast of Colombia. Multivariate data analysis was used to correlate the chemical fingerprints of soft corals with their cytotoxic activity against tumor cell lines for anticancer purpose. Some diterpenoids were identified as specific markers to discriminate between cytotoxic and non-cytotoxic crude extracts of soft corals against tumor cell lines. In the models generated from the comparative analysis of PLS-DA for tumor lines, A549 and SiHa, the diterpene 13-keto-1,11-dolabell-3(E),7(E),12(18)-triene yielded a high score in the variable importance in projection. These results highlight the potential of metabolomic approaches towards the identification of cytotoxic agents against cancer of marine origin. This workflow can be useful in several studies, mainly those that are time consuming, such as traditional bioprospecting of marine natural products.
Project description:The soft coral Scleronephthya gracillimum is an azooxanthellate octocoral order Alcyonacea. In this study, stress responses to increased seawater temperature and marine acidification were investigated using a microarray. The S. gracillimum microarray was constructed. The S. gracillimum microarray was constructed after RNA-seq. Oligonucleotides were picked from UniGene of S. gracillimum and the clones were annotated using Blast.