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Tcf1 and Lef1 regulate follicular helper T cell function eliciated by vaccination [ATAC-Seq]

ABSTRACT: Tcf1 and Lef1 regulate follicular helper T cell function eliciated by vaccination [ATAC-Seq]

PROVIDER: PRJNA610554 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11244246_1.fastq.gz	Fastqsanger.gz
	SRR11244246_2.fastq.gz	Fastqsanger.gz
	SRR11244247_1.fastq.gz	Fastqsanger.gz
	SRR11244247_2.fastq.gz	Fastqsanger.gz
	SRR11244248_1.fastq.gz	Fastqsanger.gz

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Tcf1 and Lef1 regulate follicular helper T cell function eliciated by vaccination [ATAC-Seq]

Project description:The goal of this study is to determine the impact of Tcf1 and Lef1 deficiency on T follicular helper (Tfh) cells elicited by protein immunization. We demonstrate that although phenotypic Tcf1/Lef1-deficient Tfh cells were able to develop in response to vaccination, they are functionally impaired. Mechanisc studies reveal the direct regulatory effect by Tcf1 and Lef1 on transcriptome and chromatin accessibility in vaccine-elicited Tfh cells. Analysis of chromatin accessibility in WT and Tcf1/Lef1-deficient Tfh cells eliciated by vaccination.

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Tcf1 and Lef1 regulate follicular helper T cell function eliciated by vaccination [CUTnRUN]

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Tcf1 and Lef1 regulate follicular helper T cell function eliciated by vaccination

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Tcf1 and Lef1 regulate follicular helper T cell function eliciated by vaccination [CUTnRUN]

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Impact of Tcf1 and Lef1 deficiency on histone marks and Tcf1 binding in CD8 T cells

Project description:Comparison of epigenome and Tcf1 occupancy between control and Tcf1/Lef1-deficient CD8 T cells Control mice or those are deficient for Tcf1 and Lef1 transcription factors (deleted by CD4-Cre) were used to isolate thymocytes. The thymocytes were surface-stained to identify TCRbeta high, CD69â, CD24â CD8+ subsets. These cells were sorted for ChIPseq analysis of various histone marks. Control mice or those are deficient for Tcf1 (deleted by CD4-Cre) were used to isolate thymocytes. The splenocytes were surface-stained to identify TCRbeta high, CD8+ subsets. These cells were sorted for ChIPseq analysis of Tcf1 binding locations.

2016-02-12 | E-GEOD-73239 | biostudies-arrayexpress

Impact of Tcf1 and Lef1 deficiency on mature CD8 thymocytes

Project description:Comparison of transcriptome between control and Tcf1/Lef1-deficient mature CD8 thymocytes Control mice or those are deficient for Tcf1 and Lef1 transcription factors (deleted by CD4-Cre) were used to isolate thymocytes. The thymocytes were surface-stained to identify TCRbeta high, CD69â, CD24â CD8+ subsets. These cells were sorted for RNAseq analysis.

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