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BRD9 is a critical regulator of androgen receptor signaling and prostate cancer progression - RNA-Seq

ABSTRACT: BRD9 is a critical regulator of androgen receptor signaling and prostate cancer progression - RNA-Seq

PROVIDER: PRJNA611535 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11268469_1.fastq.gz	Fastqsanger.gz
	SRR11268469_2.fastq.gz	Fastqsanger.gz
	SRR11268470_1.fastq.gz	Fastqsanger.gz
	SRR11268470_2.fastq.gz	Fastqsanger.gz
	SRR11268471_1.fastq.gz	Fastqsanger.gz

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BRD9 is a regulator of androgen receptor signaling and prostate cancer progression

Project description:BRD9 is a regulator of androgen receptor signaling and prostate cancer progression

| PRJNA611525 | ENA

BRD9 Is a Critical Regulator of Androgen Receptor Signaling and Prostate Cancer Progression.

Project description:Switch/sucrose-nonfermentable (SWI/SNF) chromatin-remodeling complexes are critical regulators of chromatin dynamics during transcription, DNA replication, and DNA repair. A recently identified SWI/SNF subcomplex termed GLTSCR1/1L-BAF (GBAF; or "noncanonical BAF", ncBAF) uniquely contains bromodomain-containing protein BRD9 and glioma tumor suppressor candidate region 1 (GLTSCR1) or its paralog GLTSCR1-like (GLTSCR1L). Recent studies have identified a unique dependency on GBAF (ncBAF) complexes in synovial sarcoma and malignant rhabdoid tumors, both of which possess aberrations in canonical BAF (cBAF) and Polybromo-BAF (PBAF) complexes. Dependencies on GBAF in malignancies without SWI/SNF aberrations, however, are less defined. Here, we show that GBAF, particularly its BRD9 subunit, is required for the viability of prostate cancer cell lines in vitro and for optimal xenograft tumor growth in vivo. BRD9 interacts with androgen receptor (AR) and CCCTC-binding factor (CTCF), and modulates AR-dependent gene expression. The GBAF complex exhibits overlapping genome localization and transcriptional targets as bromodomain and extraterminal domain-containing (BET) proteins, which are established AR coregulators. Our results demonstrate that GBAF is critical for coordinating SWI/SNF-BET cooperation and uncover a new druggable target for AR-positive prostate cancers, including those resistant to androgen deprivation or antiandrogen therapies. SIGNIFICANCE: Advanced prostate cancers resistant to androgen receptor antagonists are still susceptible to nontoxic BRD9 inhibitors, making them a promising alternative for halting AR signaling in progressed disease.

| S-EPMC8026650 | biostudies-literature

BRD9 is a regulator of androgen receptor signaling and prostate cancer progression - ChIP-Seq

Project description:BRD9 is a regulator of androgen receptor signaling and prostate cancer progression - ChIP-Seq

| PRJNA611536 | ENA

BRD9 is a regulator of androgen receptor signaling and prostate cancer progression - ChIP-Seq

Project description:BRD9 and BRD4 ChIP-Seq in LNCaP prostate cancer cells

2020-12-28 | GSE146623 | GEO

BRD9 is a critical regulator of androgen receptor signaling and prostate cancer progression - RNA-Seq

Project description:Standard RNA-Seq analysis from LNCaP cells treated with shBRD9 for 3d, shBRD9 for 11d, or 3 µM IBRD9 for 3d.

2020-12-28 | GSE146624 | GEO

BRD9 is a regulator of androgen receptor signaling and prostate cancer progression

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-12-28 | GSE146625 | GEO

Genome-wde mapping of BRD9 binding sites and gene expression changes upon BRD9 knockdown

Project description:Leukemia is characterized by genetic and epigenetic mutations resulting in selection of cancer cells, which are unable to differentiate. While genetic alterations are difficult to target, the epigenome is intrinsically dynamic and readily offers new therapeutic strategies. Thus, identifying cancer-specific context-dependent targets and unraveling their biological function may open up new therapeutic perspectives. Here, we identify bromodomain-containing protein 9 (BRD9) as a critical target required in acute myeloid leukemia (AML). We show that BRD9 is overexpressed in AML cells including ex vivo primary blasts compared to CD34+ cells. By targeting BRD9 expression in AML, we observed an alteration in proliferation and survival, ultimately resulting in the induction of apoptosis. Intriguingly, genome-wide profiling revealed that BRD9 binds enhancer regions in a cell type-specific manner, regulating cell type-related processes. We unveil a novel BRD9-sustained STAT5 pathway activation via regulation of SOCS3 expression levels. Our findings identify a previously undescribed BRD9-STAT5 axis as critical for leukemia maintenance, suggesting BRD9 as a potential therapeutic target.

2019-05-02 | GSE129437 | GEO

BRD9 regulates leukemic cell survival via STAT5 pathway inhibition

Project description:Leukemia is characterized by genetic and epigenetic mutations resulting in selection of cancer stem cells, which are unable to differentiate. While genetic alterations are difficult to target, the epigenome is intrinsically dynamic and readily offers new therapeutic strategies. Thus, identifying cancer-specific context-dependent targets and unraveling their biological function may open up new therapeutic perspectives. Here, we identify bromodomain-containing protein 9 (BRD9) as a critical target required in acute myeloid leukemia (AML). We show that BRD9 is overexpressed in AML cells including ex vivo primary blasts compared to CD34+. By targeting BRD9 expression in AML, we observed an alteration in proliferation and survival, ultimately resulting in the induction of apoptosis. Intriguingly, genome-wide profiling revealed that BRD9 binds enhancer regions in a cell type-specific manner, regulating cell type-related processes. We unveil a novel BRD9-sustained STAT5 pathway activation via regulation of SOC3 expression levels. Our findings identify a previously undescribed BRD9-STAT5 axis as critical for leukemia maintenance, suggesting BRD9 as a potential therapeutic target.

2019-11-11 | PXD013245 | Pride

Kaiso, a critical regulator of spermatogenesis

Project description:Kaiso, a critical regulator of spermatogenesis

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Androgen-sensitive glycomembrane protein expression in LNCaP prostate tumor cells

Project description:Aberrant androgen receptor (AR)-mediated transcription is a critical driver in progression of human prostate cancer. It's known that different doses of androgens can elicit differential transcriptional and proliferative responses in prostate-tumor cells. Here, we set out to examine the androgenic regulation of glycoprotein expression in the membrane fraction of prostate-tumor cells that could serve as mediators or markers of androgen-induced proliferative responses observed in prostate-tumor cells. A bioanalytical workflow involving lectin-affinity chromatography and label-free quantitative mass spectrometry was used to identify androgen-sensitive glycomembrane protein expression associated with androgen-mediated proliferation. This study would facilitate the identification of surface membrane proteins involved in androgen-mediated proliferation and provide potential therapeutic targets in the detection treatment of proliferation prostate-tumors.

2022-03-01 | PXD002913 | Pride

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