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RNA-sequencing of C3H/10T1/2 mesenchymal stem cells expressing H2B mutant histones

ABSTRACT: RNA-sequencing of C3H/10T1/2 mesenchymal stem cells expressing H2B mutant histones

PROVIDER: PRJNA612510 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11305400.fastq.gz	Fastqsanger.gz
	SRR11305401.fastq.gz	Fastqsanger.gz
	SRR11305402.fastq.gz	Fastqsanger.gz
	SRR11305403.fastq.gz	Fastqsanger.gz
	SRR11305404.fastq.gz	Fastqsanger.gz

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Expression data from wild-type and H2B K37A mutant yeast histones

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RNA-sequencing of C3H/10T1/2 mesenchymal stem cells expressing H2B mutant histones

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Gene expression profiling on C3H/10T1/2 fibroblasts during their myogenic conversion in case of Six4 ablation

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Gene expression profiling on C3H/10T1/2 fibroblasts during their myogenic conversion in case of Six4 ablation

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Project description:Imp9 is the primary importin for shuttling H2A-H2B from the cytoplasm to the nucleus. It employs an unusual mechanism where the binding of RanGTP is insufficient to release H2A-H2B. The resulting stable RanGTP•Imp9•H2A-H2B complex gains nucleosome assembly activity with H2A-H2B able to be deposited into an assembling nucleosome in vitro. Using hydrogen-deuterium exchange coupled with mass spectrometry (HDX), we show that Imp9 stabilizes H2A-H2B beyond the direct binding site, like other histone chaperones. HDX also shows that binding of RanGTP releases H2A-H2B contacts at Imp9 HEAT repeats 4-5, but not 18-19. DNA- and histone-binding surfaces of H2A-H2B are exposed in the ternary complex, facilitating nucleosome assembly. We also reveal that RanGTP has a weaker affinity for Imp9 when H2A-H2B is bound. Imp9 thus provides a connection between the nuclear import of H2A-H2B and its deposition into chromatin.

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C3H-HeH

Project description:C3H-HeH

| PRJEB10874 | ENA