Project description:To understand the molecular mechanisms of Suaeda salsa under salt stress, RNA-seq analysis was used to identify genes expressed in Suaeda salsa during salt stress response.
Project description:Arundo donax L. is one of the most promising bioenergy crop due to its high biomass yield and low irrigation requirement. The resistance to biotic and abiotic stress causes the high invasiveness of this plant, which can grow with very low management input (e.g., pesticides, fertilization, irrigation) even in marginal lands or in fields irrigated with waste or salty water. We report the leaf transcriptome sequencing, de novo assembly and annotation of a giant reed G34 genotype under salt stress. This genotype shows a different transcriptomic response to salinity compared to other A. donax genotypes. This finding was unexpected considering that the genetic variability of this species is supposed to be low due to its vegetative reproductive process. This study aims to direct future efforts towards the A. donax genetic improvement.
Project description:This study reports the first water-stressed transcriptome of Arundo donax L. (giant reed), a wild species that is emerging as one of the most promising biomass/bionergy species in mediterranean climates. Synchronized cohorts of giant reads cutting grown in hydroponic culture were subjected to water stress by addition of 10% or 20% polyethylene glycol to the roots. Shoot and root amples were collected one hour after stress. Untreated controls were collected at the same time point for shoot and root.
