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Acetobacter pomorum

ABSTRACT: Acetic acid producing bacterium

PROVIDER: PRJNA61467 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR10917701_1.fastq.gz	Fastqsanger.gz
	SRR10917701_2.fastq.gz	Fastqsanger.gz

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Acetobacter aceti

Project description:Acetic acid producing bacterium

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Transcriptome response to different carbon sources in Acetobacter aceti

Project description:Acetic acid bacteria are obligately aerobic alphaproteobacteria that have a unique ability to incompletely oxidize various alcohols and sugars to organic acids. The ability of these bacteria to incompletely oxidize ethanol to acetate has been historically utilized for vinegar production. The mechanism of switching between incomplete oxidation and assimilatory oxidation and the control of energy and carbon metabolism in acetic acid bacteria are not fully understood. To understand the physiology and molecular biology of acetic acid bacteria better, we determined the draft genome sequence of Acetobacter aceti NBRC 14818, which is the type strain of the genus. Based on this draft genome sequence, the transcriptome profiles in A. aceti cells grown on ethanol, acetate, glucose, or mix of ethanol and glucose was determined by using NimbleGen Prokaryotic Expression array (4x72K).

2011-01-17 | GSE24361 | GEO

Transcriptome response to different carbon sources in Acetobacter aceti

2011-01-17 | E-GEOD-24361 | biostudies-arrayexpress

Acetobacter pomorum

Project description:Acetobacter pomorum Genome sequencing and assembly

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Acetobacter pomorum DM001

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Ratio between Lactobacillus plantarum and Acetobacter pomorum on the surface of Drosophila melanogaster adult flies depends on cuticle melanisation.

Project description:ObjectivesAs in most organisms, the surface of the fruit fly Drosophila melanogaster is associated with bacteria. To examine whether this association depends on cuticle quality, we isolated and quantified surface bacteria in normal and melanized flies applying a new and simple protocol.ResultsOn wild flies maintained in the laboratory, we identified two persistently culturable species as Lactobacillus plantarum and Acetobacter pomorum by 16S rDNA sequencing. For quantification, we showered single flies for DNA extraction avoiding the rectum to prevent contamination from the gut. In quantitative PCR analyses, we determined the relative abundance of these two species in surface wash samples. On average, we found 17-times more A. pomorum than L. plantarum. To tentatively study the importance of the cuticle for the interaction of the surface with these bacteria, applying Crispr/Cas9 gene editing in the initial wild flies, we generated flies mutant for the ebony gene needed for cuticle melanisation and determined the L. plantarum to A. pomorum ratio on these flies. We found that the ratio between the two bacterial species reversed on ebony flies. We hypothesize that the cuticle chemistry is crucial for surface bacteria composition. This finding may inspire future studies on cuticle-microbiome interactions.

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Acetobacter pomorum DSM 11825

Project description:Acetobacter pomorum DSM 11825 genome sequencing project

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Acetobacter pomorum strain:DmCS_004

Project description:Acetobacter pomorum strain:DmCS_004 Genome sequencing and assembly

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Lactiplantibacillus pentosus

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