Project description:In order to identify RBMS1-dependent changes in RNA stability, RBMS1 levels in SW480 breast cancer cells were stably knocked-down using short-hairpin RNAs. The cells were then treated with alpha-amanitin to inhibit transcription, RNA was isolated at 0 and 9 hours post-alpha-amanitin treatment, and the samples were transcriptomically profiled.
Project description:To assess how dpeletion of RBMS1 affects the proteome, SW480 colon adenocarcinoma cells expressing control or RBMS1 shRNAs were compared by Tandem Mass Tagging (TMT) protoemics.
Project description:Knockdown of RBMS1 in pre-adipocytes impacted the differentiation process and reduced the expression of some of the key adipogenic markers. Transcriptomic and proteomic analysis indicated that RBMS1 depletion affected the expression of several genes involved in major metabolic processes, including carbohydrate and lipid metabolism.
Project description:In order to profile the translation events that were regulated by RBMS1, Ribo-seq was performed on A549 cells which was used to generate RBMS1 stably depleted and overexpressed lung cancer cells.