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MiRNA in BEAS-2B lung epithelial cells response to PRMT1 in vitro

ABSTRACT: miRNA in BEAS-2B lung epithelial cells response to PRMT1 in vitro

PROVIDER: PRJNA616322 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11452676.fastq.gz	Fastqsanger.gz
	SRR11452677.fastq.gz	Fastqsanger.gz
	SRR11452678.fastq.gz	Fastqsanger.gz
	SRR11452679.fastq.gz	Fastqsanger.gz
	SRR11452680.fastq.gz	Fastqsanger.gz

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miRNA in BEAS-2B lung epithelial cells response to PRMT1 in vitro

Project description:Our previous studies proved that both epithelial inflammation and sub-epithelial remodeling were linked to deregulated PRMT1 expression in asthma patients. This sncRNA-seq data from the BEAS-2B cell line after over-expressed PRMT1 for 24h.

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high-throughput sequencing of circRNAs in T-BEAS-2B cells and C-BEAS-2B cells

Project description:We report the differential expression of circRNAs between T-BEAS-2B cells (cadmium-transformed BEAS-2B cells) and C-BEAS-2B cells (passage-matched control BEAS-2B cells) by high-throughput sequencing. T-BEAS-2B cells are BEAS-2B cells transformed by cadmium at 2.0 μM for twenty weeks, and C-BEAS-2B cells are their passage-matched control. RNAs were sequenced on Illumina HiSeq Xten platform in triplicates, and expressions of circRNAs were calculated by TPM (transcripts per kilobase of exon model per million mapped reads). Clean data per sample exceeds 10 GB. We find 235 significantly up-regulated circRNAs and 271 significantly down-regulated circRNAs in T-BEAS-2B cells relative to C-BEAS-2B cells. Our work provides clues and evidence for exploring the mechanism of circRNAs in cadmium carcinogenesis.

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Project description:We reported the application of next generation sequencing technology for high-throughput profiling of miRNA expression in bronchile epithelial cell line Beas-2b with epithelial or mesenchymal mophology. By comparation the expression aboundence of known miRNAs between epithelial type and mesenchymal type Beas-2b cells, we found both upregulated and downregulated miRNAs in bronchial epithelial cells during EMT. This study provides a basic condition for further investigation of the roles of the regulated miRNAs during EMT in bronchial epithelial cells.

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Expression data from BEAS-2B cells

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Proteome analysis of Beas-2B cells with S. pneumoniae D39

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Expression data from BEAS-2B cells II

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Project description:Human bronchial epithelial cell line Beas-2B

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