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Trichoderma strictipile strain:TUCIM 383 | isolate:CBS 347.93

ABSTRACT: Trichoderma strictipile TUCIM 383 Transcriptome - T.strictipile

PROVIDER: PRJNA619607 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11649430_1.fastq.gz	Fastqsanger.gz
	SRR11649430_2.fastq.gz	Fastqsanger.gz

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Transcription profiling by array of Burkholderia lata strains 383 and 383-CMIT treated with preservatives

Project description:25 ml of a modified basal salts medium (BSM; Hareland et al. 1975, J Bacteriol 121:272) supplemented with 0.05% casamino acids (Becton & Dickinson, Sparks, USA), 0.05 % yeast extract (Oxoid, Basingstoke, UK) and 0.4 % glucose (Fisher Scientific, Loughborough, UK) flasks were inoculated with 2 x 10^8 CFU and incubated at 30M-0C on an orbital shaker at 150 rpm. Growth was monitored spectrophotometrically. Burkholderia lata strains evaluated were: strain 383 (LMG22485) and strain 383-CMIT (a spontaneous mutant with increased tolerance to a commercially available blend of 3:1 methylisothiazolinone & chloromethylisothiazolinone preservatives). Sub-inhibitory concentrations of preservatives added at the time of inoculation were: 0.003% dimethylol dimethyl hydantoin (DDH, Lonza Ltd, Basal, Switzerland) or 0.01 % methylisothiazolinone/chloromethylisothiazolinone (MIT/CMIT, Romm & Haas, Coventry, UK). Only B. lata strain 383 was cultured with dimethylol dimethyl hydantoin. Strain 383 cultivated without preservatives was used as control condition.<br>Cultures were harvested at mid-exponential growth phase (optical density of 0.5, 2 x 10^8 CFU), promptly aliquoted into a microcentrifuge tube and immediately snap-cooled in liquid nitrogen before centrifuging at 20.000 x g at 4M-:C for 1 min. Pellets were immediately frozen at -80M-:C.<br>

2011-07-20 | E-MEXP-2827 | biostudies-arrayexpress

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