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Novel circRNA_103758-miRNA-19b-3p-CIT axis is associated with proliferation and migration of bladder cancer via RhoA-ROCK signaling pathway [microarray]

ABSTRACT: Novel circRNA_103758-miRNA-19b-3p-CIT axis is associated with proliferation and migration of bladder cancer via RhoA-ROCK signaling pathway [microarray]

PROVIDER: PRJNA622743 | ENA |

REPOSITORIES: ENA

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Novel circRNA_103758-miRNA-19b-3p-CIT axis is associated with proliferation and migration of bladder cancer via RhoA-ROCK signaling pathway

Project description:Novel circRNA_103758-miRNA-19b-3p-CIT axis is associated with proliferation and migration of bladder cancer via RhoA-ROCK signaling pathway

| PRJNA622741 | ENA

Novel circRNA_103758-miRNA-19b-3p-CIT axis is associated with proliferation and migration of bladder cancer via RhoA-ROCK signaling pathway [RNA-seq]

Project description:Novel circRNA_103758-miRNA-19b-3p-CIT axis is associated with proliferation and migration of bladder cancer via RhoA-ROCK signaling pathway [RNA-seq]

| PRJNA622744 | ENA

Novel circRNA_103758-miRNA-19b-3p-CIT axis is associated with proliferation and migration of bladder cancer via RhoA-ROCK signaling pathway

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-08-05 | GSE147985 | GEO

miR-19b-3p Overexpression in Human Skeletal Muscle Cells

Project description:The object of this data set was to indentify genes altered by miR-19b-3p in differentiated human skeletal muscle myotubes.

2020-01-01 | GSE126187 | GEO

Novel circRNA_103758-miRNA-19b-3p-CIT axis is associated with proliferation and migration of bladder cancer via RhoA-ROCK signaling pathway [microarray]

Project description:To determine the circRNA expression profile in BCa and matched non-tumor tissues, we uesed circRNA microArray analysis form Arraystar to examine the expression of circRNAs in BCa and matched non-tumor tissues.

2020-08-05 | GSE147984 | GEO

Novel circRNA_103758-miRNA-19b-3p-CIT axis is associated with proliferation and migration of bladder cancer via RhoA-ROCK signaling pathway [RNA-seq]

Project description:Purpose:The purpose of this study is to investigate the potential mechanisms that triggering the onset of bladder cancer and to screen differential expressed genes. Methods: We screened differentially expressed genes with the data obtained from RNA-seq on Bladder carcinoma samples and matched normal samples. Functional annotation analysis and protein-protein interaction (PPI) network were performed to investigate the potential key pathways and genes that play significant roles in BCa. Furthermore, the ceRNA network was established and the Gene Set Enrichment Analysis (GSEA) was executed to dig key circRNAs. Results: Following the identification of differentially expressed genes, functional annotation analysis was conducted, suggesting that differentially expressed mRNAs (DEMs) were mainly involved in cell cycle related pathways. The top 11 genes with the highest degree were extracted from PPI network. The predicted regulatory relationships among the differentially expressed genes were found and the ceRNA network was established. Finally, GSEA was conducted on the circRNAs and the key biological pathways were identified. Conclusion: Our study is the pioneering research to analyze the pathogenesis of BCa with integrated informatics and we have identified several hub genes, which reveals that the generation of BCa is closely related to cell cycle.

2020-08-05 | GSE147983 | GEO

miR-19b-3p Overexpression in Human Skeletal Muscle Cells

Project description:miR-19b-3p Overexpression in Human Skeletal Muscle Cells

| PRJNA521255 | ENA

Novel circRNA_0071196/miRNA‑19b‑3p/CIT axis is associated with proliferation and migration of bladder cancer.

Project description:Circular RNAs (circRNAs) are non‑coding RNAs that are connected at the 3' and 5' ends by an exon or intron. Studies increasingly show that circRNAs play an important role in tumorigenesis by acting as a 'sponge' for microRNAs (miRNAs), which abrogates the latter's effect on their target mRNAs. To identify a possible circRNA/miRNA/mRNA network in bladder cancer (BCa), we analyzed the circRNA and mRNA expression profiles of BCa and adjacent normal bladder tissues. A total of 127 circRNAs and 1,612 mRNAs were differentially expressed in the tumor tissues, and were primarily associated with cancer‑related pathways. A competing endogenous RNAs (ceRNA) network was then constructed which predicted a regulatory axis of circRNA_0071196, miRNA‑19b‑3p and its target gene citron Rho‑interacting serine/threonine kinase (CIT). Luciferase reporter assay validated the relationship between circRNA_0071196 and miRNA‑19b‑3p and of the latter with CIT. Furthermore, CIT was overexpressed in the BCa tissues, and was found to be correlated with metastasis and tumor histological grade. Knockdown of CIT in the human bladder cancer cell line 5367 significantly inhibited the proliferation, migration and colony formation capacity of the cells, and also upregulated the mediators of the p53 and RhoA‑ROCK signaling cascades that regulate cell cycle and migration. Taken together, our findings indicate that circRNA‑0071196 upregulates CIT levels in BCa by sponging off miRNA‑19b‑3p, and the circRNA_0071196/miRNA‑19b‑3p/CIT axis is a potential therapeutic target in BCa.

| S-EPMC7384843 | biostudies-literature

RhoA-ROCK competes with YAP to regulate amoeboid breast cancer cell migration in response to lymphatic-like flow

Project description:Lymphatic drainage generates force that induces prostate cancer cell motility via activation of Yes-associated protein (YAP), but whether this response to fluid force is conserved across cancer types is unclear. Here, we show that shear stress corresponding to fluid flow in the initial lymphatics modifies taxis in breast cancer. Whereas some cell lines employ rapid amoeboid migration behavior in response to fluid flow, a separate subset decrease movement. Positive responders displayed transcriptional profiles typical of an amoeboid cell state. Regulation of the HIPPO tumor suppressor pathway and YAP activity also differed between breast subsets and prostate cancer. Although subcellular localization of YAP to the nucleus positively correlated with overall velocity of locomotion, YAP gain- and loss-of-function demonstrates that YAP inhibits breast cancer motility but is outcompeted by other pro-taxis mediators in the context of flow. Specifically, we show that RhoA dictates response to flow. GTPase activity of RhoA, but not Rac1 or Cdc42 Rho family GTPases, is elevated in cells that positively respond to flow and is unchanged in cells that decelerate under flow. Disruption of RhoA or the RhoA effector, Rho-associated kinase (ROCK), blocked shear stress-induced motility. Collectively, these findings demonstrate stratification of breast cancer subsets by flow-sensing mechanotransduction pathways and point to a role for biophysical force in regulation of an amoeboid cell state.

2022-03-27 | GSE191142 | GEO

Loss of NUMB drives aggressive bladder cancer via a RHOA/ROCK/YAP signaling axis.

Project description:We have discovered a previously uncharacterized tumor suppressor function of Numb in the homeostasis of the normal bladder mucosa. Targeted ablation of Numb in the basal layer of the urothelium drives spontaneous bladder tumorigenesis, driving progression from preneoplastic to preinvasive and overtly invasive tumors, and accelerates tumorigenesis in the presence of oncogenic insults. Using 3D-Matrigel organoid cultures to recapitulate bladder tumorigenesis in vitro, we found that Numb deficiency enhances the proliferative and invasive potential of both mouse and human bladder cancer cells. Integrative transcriptomic and functional analyses revealed that downregulation of the canonical Hippo pathway, resulting in enhanced YAP transcriptional activity, underlies the biological aggressiveness of Numb-deficient bladder cancer. These molecular events are dependent on the activation of RhoA/ROCK signaling subsequent to Numb loss. Thus, a dysfunctional Numb–RhoA/ROCK–Hippo/YAP regulatory network is at play in aggressive Numb-deficient bladder cancers, opening avenues for the development of targeted therapies. Furthermore, retrospective cohort studies revealed that a Numb-deficient status correlates with worse overall survival in post-cystectomy muscle-invasive bladder cancer (MIBC) patients and increased risk of progression to MIBC in non-muscle-invasive bladder cancer (NMIBC) patients. The Numb-deficient status was associated with a 27-gene prognostic signature that has the potential to identify high-risk NMIBC patients who are eligible for targeted RhoA/ROCK/YAP therapies.

2024-10-11 | GSE256272 | GEO

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