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EPR (BC030870) ChIP-Seq analysis in NMuMG mouse mammary gland

ABSTRACT: EPR (BC030870) ChIP-Seq analysis in NMuMG mouse mammary gland

PROVIDER: PRJNA623393 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11490741.fastq.gz	Fastqsanger.gz
	SRR11490742.fastq.gz	Fastqsanger.gz
	SRR11490743.fastq.gz	Fastqsanger.gz
	SRR11490744.fastq.gz	Fastqsanger.gz
	SRR11490745.fastq.gz	Fastqsanger.gz

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EPR (BC030870) ChIP-Seq analysis in NMuMG mouse mammary gland

Project description:EPR, a lncRNA present in the chromatin fraction of NMuMG cells, controls proliferation and fate determination in mammary gland cells. We wanted to define if EPR overexpression in NMuMG cells affects the landcape of two histone activation marks (H3K4me3 and H3K27ac).

2020-04-07 | GSE148182 | GEO

EPR (BC030870) ChIRP-Seq analysis in NMuMG mouse mammary gland

Project description:EPR, a lncRNA present in the chromatin fraction of NMuMG cells, controls proliferation and fate determination in mammary gland cells. We wanted to identify its genomic targets. We identified thousands of targets, many of them located in regulatory regions.

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EPR (BC030870) ChIRP-Seq analysis in NMuMG mouse mammary gland

Project description:EPR (BC030870) ChIRP-Seq analysis in NMuMG mouse mammary gland

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ChIP-Seq of Klf4 in NMuMG normal murine mammary gland epithelial cells.

Project description:Chromatin immunoprecipitation of Klf4 in NMuMG cells identifies gene promoter sequences that are directly bound by Klf4. Chromatin immunoprecipitation using a Klf4-specific antibody in NMuMG cells followed by next generation sequencing (ChIP-Seq).

2013-08-01 | E-GEOD-49445 | biostudies-arrayexpress

LncRNA EPR-induced METTL7A1 modulates target gene translation

Project description:EPR is a long non-coding RNA (lncRNA) that controls cell proliferation in mammary gland cells by regulating gene transcription. Here, we report on Mettl7a1 as a direct target of EPR. We show that EPR induces Mettl7a1 transcription by rewiring three-dimensional chromatin interactions at the Mettl7a1 locus. Our data indicate that METTL7A1 contributes to EPR-dependent inhibition of TGF-β signaling. METTL7A1 is absent in tumorigenic murine mammary gland cells and its human ortholog (METTL7A) is downregulated in breast cancers. Importantly, re-expression of METTL7A1 in 4T1 tumorigenic cells attenuates their transformation potential, with the putative methyltransferase activity of METTL7A1 being dispensable for its biological functions. We found that METTL7A1 localizes in the cytoplasm whereby it interacts with factors implicated in the early steps of mRNA translation, associates with ribosomes, and affects the levels of target proteins without altering mRNA abundance. Overall, our data indicates that METTL7A1 —a transcriptional target of EPR— modulates translation of select transcripts.

2022-08-12 | PXD034398 | Pride

Smad2/3 binding regions in NMuMG cells

Project description:Smad2/3 binding regions in mouse mammary gland epithelial cells (NMuMG) treated with TGF-beta for 1.5 h were determined by ChIP-seq to evaluate the transcriptional mechanism of TGF-beta-Smad signaling.

2019-08-29 | GSE121254 | GEO

Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes (Polysomes)

Project description:EPR is a long non-coding RNA (lncRNA) that controls cell proliferation in mammary gland cells by regulating gene transcription and, here, we report on Mettl7a1 as a target of EPR. We show that the lncRNA induces Mettl7a1 transcription by remodeling the 3-dimensional chromatin structure at the Mettl7a1 locus. Our data indicate that METTL7A1 participates in the EPR-dependent pathway that antagonizes TGF-β signaling. METTL7A1 is absent in tumorigenic murine mammary gland cells and its human ortholog (METT7A) is downregulated in breast cancers. Importantly, expression of METTL7A1 in 4T1 tumorigenic cells reduces their transformation potential, and the putative methyltransferase activity of METTL7A1 appears dispensable for its biological functions. METTL7A1 is a cytoplasmic protein and, from a mechanistic perspective, interacts with factors implicated in the early steps of mRNA translation, associates with ribosomes, and affects the levels of select proteins without substantial changes in mRNA abundance. Our data suggest the possibility that METTL7A1 conveys the transcriptional regulation operated by EPR into specific changes of mRNA translation.

2022-05-28 | GSE195821 | GEO

Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes (Total RNA)

2022-05-28 | GSE195822 | GEO

Identification of a LncRNA EPR—>METTL7A1 pathway modulating translation of select genes (3C-Seq)

2022-05-28 | GSE195819 | GEO

ChIP-Seq of Klf4 in NMuMG normal murine mammary gland epithelial cells.

Project description:Chromatin immunoprecipitation of Klf4 in NMuMG cells identifies gene promoter sequences that are directly bound by Klf4.

2013-08-01 | GSE49445 | GEO