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Titanium Tackles the Endolplasmic Reticulum: A First Genomic Study on a Titanium Anticacner Metallodrug

ABSTRACT: Titanium Tackles the Endolplasmic Reticulum: A First Genomic Study on a Titanium Anticacner Metallodrug

PROVIDER: PRJNA623570 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11498104.fastq.gz	Fastqsanger.gz
	SRR11498105.fastq.gz	Fastqsanger.gz
	SRR11498106.fastq.gz	Fastqsanger.gz
	SRR11498107.fastq.gz	Fastqsanger.gz
	SRR11498108.fastq.gz	Fastqsanger.gz

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Titanium Tackles the Endolplasmic Reticulum: A First Genomic Study on a Titanium Anticacner Metallodrug

Project description:PhenolaTi is novel non-toxic anticancer chemotherapy; this inert bis(phenolato)bis(alkoxo) Ti(IV) complex demonstrates the intriguing combination of high and wide efficacy with no detected toxicity in animals. Here we unravel the cellular pathways involved in its mechanism of action by a first genome study on Ti(IV)-treated cells, using an attuned RNA-Seq-based available technology. First, phenolaTi induced apoptosis and cell-cycle arrest at the G2/M phase in MCF7 cells. Second, the transcriptome of the treated cells was analyzed, identifying alterations in pathways relating to protein translation, DNA damage, mitochondrial eruption, and p53 regulation. Unlike for common metallodrugs, electrophoresis assay showed no inhibition of DNA polymerase activity. Reduced in vitro cytotoxicity with added endoplasmic reticulum (ER) stress inhibitor supported the ER as a putative cellular target. Altogether, this paper reveals a distinct ER-related mechanism by the Ti(IV) anticancer coordination complex, paving the way for wider applicability of related techniques in mechanistic analyses of metallodrugs.

2020-07-07 | GSE148239 | GEO

Titanium Tackles the Endoplasmic Reticulum: A First Genomic Study on a Titanium Anticancer Metallodrug.

Project description:PhenolaTi is an advanced non-toxic anticancer chemotherapy; this inert bis(phenolato)bis(alkoxo) Ti(IV) complex demonstrates the intriguing combination of high and wide efficacy with no detected toxicity in animals. Here we unravel the cellular pathways involved in its mechanism of action by a first genome study on Ti(IV)-treated cells, using an attuned RNA sequencing-based available technology. First, phenolaTi induced apoptosis and cell-cycle arrest at the G2/M phase in MCF7 cells. Second, the transcriptome of the treated cells was analyzed, identifying alterations in pathways relating to protein translation, DNA damage, and mitochondrial eruption. Unlike for common metallodrugs, electrophoresis assay showed no inhibition of DNA polymerase activity. Reduced in vitro cytotoxicity with added endoplasmic reticulum (ER) stress inhibitor supported the ER as a putative cellular target. Altogether, this paper reveals a distinct ER-related mechanism by the Ti(IV) anticancer coordination complex, paving the way for wider applicability of related techniques in mechanistic analyses of metallodrugs.

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Investigation of titanium coated imprinted polystyrene surfaces

Project description:In this study the gene expression differences between two titanium surfaces produced at Maastricht University were investigated. These two surfaces were: flat titanium-coated polystyrene and a titanium-coated polystyrene surface imprinted with a pattern selected from an earlier screening study (Ti1018). This pattern was selected based on the osteoinductive properties observed. As a positive control cells on the flat surface were treated with dexamethasone.

2018-09-25 | E-MTAB-7212 | biostudies-arrayexpress

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Project description:In this study the gene expression differences between three titanium surfaces produced by Straumann were investigated. These three surfaces were: flat pre-treated (Pt) titanium, sandblasted (S) titanium and sandblasted acid-etched (SLA) titanium. The SLA surface is known to boost the proliferation and osteogenic differentiation of MG-63 cells. SLA titanium is also widely used for dental implants.

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