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Profiling of gene expression signatures for human corneal epithelial cell after transfection with microRNA-184 or NC.

ABSTRACT: Profiling of gene expression signatures for human corneal epithelial cell after transfection with microRNA-184 or NC.

PROVIDER: PRJNA623753 | ENA |

REPOSITORIES: ENA

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Profiling of gene expression signatures for human corneal epithelial cell after transfection with microRNA-184 or NC.

Project description:Our previous study identified that microRNA-184 as one of the most highly expressed miRNAs in the corneal epithelium. To gain insight into the underlying mechanisms of miR-184 modulation of corneal epithelial cell proliferation and migration, we probed for its gene targets by microarray analysis . HCECs transfected with miR-184 or a NC for 48 h were harvested and a gene microarray evaluated transcriptome expression patterns. Ectopic miR-184 expression resulted in numerous genes undergoing either upregulation or downregulation. Since miRNAs mediate biological functions by impeding expression of their target genes in mammals, a total of 901 differentially downregulated genes (Foldchange ≤ 0.667) were firstly selected.

2020-08-11 | GSE148289 | GEO

MicroRNA-145 Regulates Human Corneal Epithelial Differentiation [Agilent-014850 array data]

2011-10-10 | GSE24980 | GEO

MicroRNA-145 Regulates Human Corneal Epithelial Differentiation [Agilent-014850 array data]

Project description:To investigate the gene expression in human corneal epithelial overexpressing hsa-miR-145 by transfection , we have employed Whole Human Genome Oligo Microarray (Agilent) as a screening platform to identify gene regulation. We discovered a differential gene expression in HCE cells transfected with hsa-mIR-145 against cells with scrambled sequences. Among them, genes related with corneal development, integrity, differentiation and inflammatory responses were found and this was validated by real-time PCR. HCE cells transfected with hsa-miR-145 or scrambled sequences were collected at 24 hours after transfection. Total RNA was extracted by Trizol/chloroform and purified with RNeasy mini spin column. RNA samples with 28S/18S ratios in the range of 1.4 to 1.8 were used for expression RNA profiling using Whole Human Genome Oligo Microarray (Agilent).

2011-10-09 | E-GEOD-24980 | biostudies-arrayexpress

microRNA Expression of mice corneal endothelia

Project description:MicroRNA profile comparison of the corneal endothelium of young and old mice: implications for senescence of the corneal endothelium We collected the corneal endothelia from 30 mice aged 10-13 weeks and the corneal endothelia from 30 mice aged 2 years. The samples were pooled into six groups (y1, y2, y3 and s1, s2, s3). Each group comprised corneal endothelia from ten mice, and these six groups were used for a genome-wide microRNA microarray study.

2013-12-27 | E-GEOD-48094 | biostudies-arrayexpress

microRNA Expression of mice corneal endothelia

Project description:MicroRNA profile comparison of the corneal endothelium of young and old mice: implications for senescence of the corneal endothelium

2013-12-27 | GSE48094 | GEO

Mouse gene expression profile after transfected with miR-27a mimics and mimic NC in Raw

Project description:Microarry analysis of mouse gene expression profile after transfected with miR-27a mimics (27a-7) and mimic NC (NC-9) Goal was to determine the effects of miR-27a transfection on global gene expression.

2013-01-31 | GSE43910 | GEO

Glaesserella sp. 15-184 strain:15-184-2

Project description:Glaesserella sp. 15-184 strain:15-184-2 Genome sequencing

| PRJNA397816 | ENA

Escherichia coli strain:184/2aE | isolate:184/2aE

Project description:Escherichia coli strain:184/2aE | isolate:184/2aE Genome sequencing

| PRJNA719994 | ENA

MicroRNA-145 Regulates Human Corneal Epithelial Differentiation

Project description:This SuperSeries is composed of the following subset Series: GSE24979: MicroRNA-145 Regulates Human Corneal Epithelial Differentiation [Agilent-016436 array data] GSE24980: MicroRNA-145 Regulates Human Corneal Epithelial Differentiation [Agilent-014850 array data] Refer to individual Series

2011-10-09 | E-GEOD-24981 | biostudies-arrayexpress

MicroRNA profiling of the developing mammary gland identifies miR-184 as a candidate breast tumour suppressor gene (miRNA)

Project description:The study of mammalian development has offered many insights into the molecular aetiology of cancer. We previously used analysis of mammary morphogenesis to discover a critical role for GATA-3 in mammary developmental and carcinogenesis. In recent years an important role for MicroRNAs (miRNAs) in a myriad of cellular processes in development and in oncogenesis has emerged. In this study, microRNA profiling of stromal and epithelial cellular subsets microdissected from the developing mouse mammary gland revealed many microRNAs with expression restricted to various cellular subsets. MicroRNA-184 (miR-184) was exclusively expressed in epithelial cells and markedly upregulated during differentiation of the proliferative, invasive cells of the pubertal terminal end bud (TEB) into ductal epithelial cells in vivo and in FACS-sorted mammary stem cells (MaSCs) versus luminal epithelial cells. miR-184 expression was silenced in mouse tumour models compared to non-transformed epithelium and in a majority of breast cancer cell line models. Ectopic reactivation of miR-184 inhibited the proliferation and self-renewal of metastatic triple negative breast cancer (TNBC) cell lines in vitro and delayed tumour formation and reduced metastasis in vivo. Gene expression studies uncovered multi-factorial direct regulation of genes in the AKT/mTORC1 pathway by miR-184. In clinical breast cancer tissues, expression of miR-184 is lost in primary TNBCs while the miR-184 promoter is methylated in a subset of lymph node metastases from TNBC patients. These studies elucidated a new layer of regulation in the PI3K/AKT/mTOR pathway with relevance to mammary development and tumour proliferation and metastasis.

2015-10-01 | GSE62260 | GEO

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