Project description:Fumarate hydratase (FH) is the enzyme in the Krebs cycle, which transforms fumarate to malate. Loss of fumarate hyrdragase leads to hereditary leiomyomatosis and renal cell cancer (HLRCC). The biallelic inactivation has been highly accumulated in FH-deficient cells and is considered a major pro-oncogenic factor for HLRCC tumorigenesis. We used microarrays to understand the global readaptation of cell metabolism of gene expression underlying the truncated Krebs cycle by loss of function of fumarate hydratase. The fumarate hydratase wild type and knock out cells were generated for RNA extraction and hybridization on Affymetrix microarrays.
Project description:Series of 6 repetitions of hybridization of treatment (pBroL) and control (pBroD) each. Comparison of plants treated with nitrile herbicide bromoxynil 30' after reexposure to light (after one 12h dark period) versus treatment with bromoxynil and incubation for 12h in darkness. E. Richly et al., EMBO Rep. 4 (2003), pp. 491–498 Keywords: repeat sample
Project description:Series of 6 repetitions of hybridization of treatment (pBro) and control (H2O) each. Comparison of plants treated with nitrile herbicide bromoxynil 30' after reexposure to light (after one 12h dark period) versus 30' after reexposure to light and treatment with H20 as control. E. Richly et al., EMBO Rep. 4 (2003), pp. 491–498 Keywords: repeat sample
2005-01-01 | GSE1200 | GEO
Project description:Identification of nitrile degrading organisms.
Project description:Series of 6 repetitions of hybridization of treatment (pBroL) and control (pBroD) each. Comparison of plants treated with nitrile herbicide bromoxynil 30' after reexposure to light (after one 12h dark period) versus treatment with bromoxynil and incubation for 12h in darkness. E. Richly et al., EMBO Rep. 4 (2003), pp. 491–498 Keywords: repeat sample
Project description:Series of 6 repetitions of hybridization of treatment (pBro) and control (H2O) each. Comparison of plants treated with nitrile herbicide bromoxynil 30' after reexposure to light (after one 12h dark period) versus 30' after reexposure to light and treatment with H20 as control. E. Richly et al., EMBO Rep. 4 (2003), pp. 491–498 Keywords: repeat sample
Project description:A series of gene expression measurements of normal myometrium and uterine fibroids with mutated or wild-type fumarate hydratase (FH) gene.
