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Locus specific epigenetic modalities of random allelic expression imbalance

ABSTRACT: Locus specific epigenetic modalities of random allelic expression imbalance

PROVIDER: PRJNA623946 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11513082_1.fastq.gz	Fastqsanger.gz
	SRR11513082_2.fastq.gz	Fastqsanger.gz
	SRR11513083_1.fastq.gz	Fastqsanger.gz
	SRR11513083_2.fastq.gz	Fastqsanger.gz
	SRR11513084_1.fastq.gz	Fastqsanger.gz

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Locus specific epigenetic modalities of random allelic expression imbalance

Project description:Genes are generally thought to be expressed from both alleles. There are some exceptions, including Random monoallelic expression (RME), even though its extent is still somewhat debated. We assessed whether and how some autosomal genes that we previously identified as being RME are prone to persistent and stable monoallelic expression. Not only do we confirm the occurrence of RME at large frequencies, our results also reveal unforeseen modes of allelic expression,that appear to be gene specific and epigenetically regulated. This non-canonical allelic regulation has large physiological and pathological implications and represents novel therapeutic perspectives.

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Locus specific epigenetic modalities of random allelic expression imbalance.

Project description:Most autosomal genes are thought to be expressed from both alleles, with some notable exceptions, including imprinted genes and genes showing random monoallelic expression (RME). The extent and nature of RME has been the subject of debate. Here we investigate the expression of several candidate RME genes in F1 hybrid mouse cells before and after differentiation, to define how they become persistently, monoallelically expressed. Clonal monoallelic expression is not present in embryonic stem cells, but we observe high frequencies of monoallelism in neuronal progenitor cells by assessing expression status in more than 200 clones. We uncover unforeseen modes of allelic expression that appear to be gene-specific and epigenetically regulated. This non-canonical allelic regulation has important implications for development and disease, including autosomal dominant disorders and opens up therapeutic perspectives.

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