Project description:The high-throughput sequencing technology was performed after the treatment of human triple negative breast cancer cells MDA-MB-231 and BT549 with Lespedeza bicolor root extracted by ourselves, to explore the expression of genes related to cell proliferation, adhesion, migration and invasion of human triple negative breast cancer cells MDA-MB-231 and BT549 after the treatment of Lespedeza bicolor root changes, and to find an interesting target gene.
Project description:Parallel Analysis of RNA Ends (PARE) sequencing reads were generated to validate putative microRNAs and identify cleavage sites in Sorghum bicolor and Setaria viridis.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Meliniomyces bicolor mycorrhizal roots compared to free-living mycelium . Mycorrhizal roots and control mycelium were harvested after 113 days and used for RNA extraction. Reads of 150bp were generated and aligned to Meliniomyces bicolor transcripts (https://genome.jgi.doe.gov/Melbi2/Melbi2.home.html) using CLC Genomics Workbench 8.