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Vibrio natriegens

ABSTRACT: Changes in Growth of Vibrio natriegens under Simulated Microgravity

PROVIDER: PRJNA624924 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11547047_1.fastq.gz	Fastqsanger.gz
	SRR11547047_2.fastq.gz	Fastqsanger.gz
	SRR11547048_1.fastq.gz	Fastqsanger.gz
	SRR11547048_2.fastq.gz	Fastqsanger.gz
	SRR11547049_1.fastq.gz	Fastqsanger.gz

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Project description:Vibrio natriegens strain ATCC 14048 is a salt marsh isolate with a notably rapid growth rate that has garnered considerable interest for biotechnological applications. We used systems-level tools (i.e. metabolomics, transcriptomics, proteomics) to characterize its physiological response to different salinities and temperatures that are relevant not only to its natural environment but also to planned scalable culturing processes. We found organic osmolyte synthesis and membrane transporters were most responsive to changes in salinity. The osmolytes glutamate, glutamine and ectoine responded to salinity across temperature treatments. However, when media was supplemented with choline, glycine betaine appeared to replace ectoine. These results provide a baseline data set for metabolic activity under a variety of conditions that will influence future basic and applied V. natriegens research.

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Project description:Vibrio natriegens is a rapidly growing salt marsh bacterium that is being developed as a synthetic biology chassis. We characterized its physiological response to different salinities and temperatures in order to optimize culturing conditions and understand its adaptations to a salt marsh environment. Using metabolomics, transcriptomics, and proteomics we determined what pathways respond to these environmental parameters. We found that organic osmolyte synthesis and membrane transporters were most responsive to changes in salinity. The primary osmolytes were glutamate, glutamine, and ectoine, responding to salinity across temperature treatments. However, when media was supplemented with choline, glycine betaine seemed to mostly replace ectoine. These results provide a baseline dataset of metabolic activity under a variety of conditions that will inform decisions made about culturing and genome engineering for future applications.

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Rationally designed chromosome fusion is not a limiting factor for rapid growth of Vibrio natriegens

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