Project description:We employed a proteogenomics workflow to identify microproteins encoded by small Open Reading Frames (ORFs) in the genome of Mycobacterium smegmatis strain mc²155.
Project description:In this approach, changes in total transcript of a glnR deletion strain of Mycobacterium smegmatis due to nitrogen starvation were monitored. This is a control experiment for the comparison of wild type and glnR deletion strain resulting in putative nitrogen-related genes which are not controlled by GlnR.
Project description:In this approach, total transcript of a Mycobacterium smegmatis amtR deletion strain was compared under nitrogen surplus and starvation. This is a control experiment to the comparison of wild type and amtR deletion strain, resulting nitrogen-related genes which are not AmtR-controlled. Two biological replicates were analyzed.