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Transcriptomic analysis of Wild-type and Mfsd7c-/-knockout brain embryos

ABSTRACT: Transcriptomic analysis of Wild-type and Mfsd7c-/-knockout brain embryos

PROVIDER: PRJNA625965 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11563211_1.fastq.gz	Fastqsanger.gz
	SRR11563211_2.fastq.gz	Fastqsanger.gz
	SRR11563212_1.fastq.gz	Fastqsanger.gz
	SRR11563212_2.fastq.gz	Fastqsanger.gz
	SRR11563213_1.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of Wild-type and Mfsd7c-/-knockout brain embryos

Project description:Mfsd7c is expressed in CNS blood vessels. Deletion of Mfsd7c results in embryonic lethality with signs of hypoxia. We performed transcriptomic analysis of genes in brains of E14.5 wild-type (WT) and Mfsd7c-knockout (KO) embryos.

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Gene expression changes in Dhcr7 knockout brain, lung and liver at E18.5 compared to wild-type embryos

Project description:The genetic defect underlying the human Smith-Lemli-Opitz dysmorphological disorder is loss-of-function mutations affecting the cholesterol synthesis enzyme dehydrocholesterol delta7 reductase, DHCR7. Dhcr7 knockout mice recapitulate the biochemical characteristics, but all knockout pups die within 14h of birth. Tissues of knockout mice accumulate the precursor sterol, 7-dehydrocholesterol, and show reduced levels of cholesterol (J. Clin. Invest. (2001) 108: 905-915). We compared the global gene expression changes in lung, liver and brain from knockout mice to those seen from organs harvested from same-pregnancy wild-type embryos, harvested before birth (E18.5). Since the P0 knockout pups die, we expected that there would be significant changes in gene expression between knockout and wild-type organs, and further comparing the altered genes in common to brain, lung and liver would point to a common mechanistic pathway where disruption of normal sterol synthesis in all cells leads to pathophysiology. Remarkably, these data show that the global gene expression between knockout and wild-type organs is hardly altered, despite the complete loss of cholesterol synthesis. There are so few genes that are altered, and furthermore, those that are altered are very limited in sharing similarities in all three tissues. This suggests that disorganized gene expression is not the cause of the early neonatal lethality. Dhcr7+/- females were mated with Dhcr+/- males, and timed pregnancy obtained. At E18.5, pregnant dams were sacrificed, and embryos harvested, organs removed and RNA extracted, and all embryos were genotyped. Matching wild-type and knockout embryos were taken from each pregnancy, and two females yielded all genotypes used herein.

2012-08-01 | E-GEOD-39811 | biostudies-arrayexpress

Gene expression changes in Dhcr7 knockout brain, lung and liver at E18.5 compared to wild-type embryos

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Expression analysis of miR-302 knockout and wild-type embryos during neurulation

Project description:Wild-type or miR-302 knockout tissue was isolated from embryos during neurulation in replicate

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Transcription profiling of mouse ADNP knockout vs wild type

Project description:The putative transcription factor, activity-dependent neuroprotective protein (ADNP) is a zinc finger and homeodomain - like profile containing protein. ADNP knockout mice die at day 9 of gestation. To reveal ADNP-associated pathways, a 22,690- Affymetrix probe array was used on ADNP knockout and control embryos. References:; Complete sequence of a novel protein containing a femtomolar-activity-dependent neuroprotective peptide. J Neurochem. 1999 Mar;72(3):1283-93. PMID: 10037502 [PubMed - indexed for MEDLINE]; Cloning and characterization of the human activity-dependent neuroprotective protein. J Biol Chem. 2001 Jan 5;276(1):708-14. PMID: 11013255 [PubMed - indexed for MEDLINE]; Activity-dependent neuroprotective protein: a novel gene essential for brain formation. Brain Res Dev Brain Res. 2003 Aug 12;144(1):83-90. PMID: 12888219 [PubMed - indexed for MEDLINE] Experiment Overall Design: To identify downstream target genes of ADNP, we examined global gene expression profiles of whole E9 knockout embryos (KO - ADNP -l-), their wild-type (WT - ADNP +l+ ) and their heterozygous (H - ADNP âl+ ) littermates, using the Affymetrix Murine Genome 430A oligonucleotide microarrays. Gestation day 9 was chosen as it is the period at which embryos lacking ADNP exhibit distinct morphological and developmental changes prior to degeneration and in uterus absorption. Total RNA was extracted from four ADNP knockout embryos, four normal embryos and six heterozygous embryos. A pool of two genotype identical littermates was used on seven different arrays (pooling of two embryos was necessary to obtain enough RNA on each of the gene microarrays).

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AQP8 proteome project - Wild type and AQP8 Knockout proteomes

Project description:Wild type and AQP8 Knockout proteomes

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