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RiboTag-IP-RNA sequencing of parvalbumin neurons in frontal cortex of Fxr1 knockout mice

ABSTRACT: RiboTag-IP-RNA sequencing of parvalbumin neurons in frontal cortex of Fxr1 knockout mice

PROVIDER: PRJNA626354 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11566986.fastq.gz	Fastqsanger.gz
	SRR11566987.fastq.gz	Fastqsanger.gz
	SRR11566988.fastq.gz	Fastqsanger.gz
	SRR11566989.fastq.gz	Fastqsanger.gz
	SRR11566990.fastq.gz	Fastqsanger.gz

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RiboTag-IP-RNA sequencing of parvalbumin neurons in frontal cortex of Fxr1 knockout mice

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Project description:The RBP, FXR1, is overexpressed in many epithelial tumors containing a canonical RGG box domain. FXR1 controls post-transcriptional gene regulation through changes in mRNA turnover and translation of target genes. Here we show that arginine methyltransferase PRMT5- mediated specific arginine methylation of FXR1 increases its stability in cancer cells. FXR1-dependent gene signatures show decreased expression and instability of G4-rich sequences containing mRNAs such as CDKN1A, PLK2, TCN2, and TRAF4. Furthermore, structural features of FXR1 and G4-RNA interactions provide novel insights into the critical arginine amino acids of FXR1 essential for G4-RNA-binding and turnover activity. In addition, analysis of the eCLIP data provides various RNA targets and the G-rich sequence motifs of FXR1 in head and neck cancer cell line. Thus, our results indicate that PRMT5-mediated methylation of FXR1 binding with G4-RNAs favors mRNA stability and turnover, contributing to cancer cell growth and proliferation.

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