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Plasmodium falciparum strain:3D7-A

ABSTRACT: Genetic variants associated with heat shock sensitivity in Plasmodium falciparum 3D7-A parasites

PROVIDER: PRJNA626524 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR11784184_1.fastq.gz	Fastqsanger.gz
	SRR11784184_2.fastq.gz	Fastqsanger.gz
	SRR11784185_1.fastq.gz	Fastqsanger.gz
	SRR11784185_2.fastq.gz	Fastqsanger.gz
	SRR11784186_1.fastq.gz	Fastqsanger.gz

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Transcriptional alterations of P. falciparum 3D7-A heat shock-adapted vs control parasites.

Project description:Periodic fever is the most characteristic clinical feature of human malaria. However, how parasites survive malarial febrile episodes, which often involve temperatures of >40ºC, is not known. To understand the molecular basis of heat shock (HS) resistance in Plasmodium falciparum, we took advantage of previously developed P. falciparum lines adapted to periodic HS (3D7-A-HS) and their non-selected controls maintained in parallel (3D7-A). Their ability to adapt in only a few cycles suggested that the parental parasite population contained a selectable subset of parasites resistant to HS. Transcriptomic analysis of both 3D7-A-HS and 3D7-A was used to study the differences at a transcriptional level that could be related with the HS-resistant phenotype.

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Expression analysis of Plasmodium falciparum 3D7 delta-PfPuf2 mutant

Project description:Investigation of whole genome gene expression level changes in Plasmodium falciparum 3D7 delta-PfPuf2 mutant, compared to the wild-type strain 3D7. The mutation engineered into this strain render tanslational control. The mutants analyzed in this study are further described in Miao J, Li J, Fan Q, Li X, Li X, Cui L.2010. The Puf-family RNA-binding protein PfPuf2 regulates sexual development and sex differentiation in the malaria parasite Plasmodium falciparum. J Cell Sci. 123(7):1039-49 (PMID 20197405). A 12 chip study using total RNA recovered from six separate wild-type cultures of Plasmodium falciparum 3D7 at gametocyte stage III (three cultures) and stage V (three cultures) and six separate cultures of dalta PfPuf2 mutant at gametocyte stage III (three cultures) and stage V (three cultures). Each chip measures the expression level of 5,367 genes from Plasmodium falciparum 3D7 with 45-60 mer probes with two replicates on final array of 71618 probes.

2012-07-05 | E-GEOD-39124 | biostudies-arrayexpress

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Project description:Plasmodium falciparum 3D7 Genome sequencing

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Project description:Plasmodium falciparum 3D7 Raw sequence reads