Aedes albopictus, Culex pipiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA626593 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR11577300_1.fastq.gz | Fastqsanger.gz | |||
| SRR11577300_2.fastq.gz | Fastqsanger.gz | |||
| SRR11577400_1.fastq.gz | Fastqsanger.gz | |||
| SRR11577400_2.fastq.gz | Fastqsanger.gz | |||
| SRR11577500_1.fastq.gz | Fastqsanger.gz |
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Project description:Investigation of gene expression level differences in Culex pipiens (SB) and C. quinquefasciatus (JHB) at three time points (8, 16, and 24 hours post-exposure) during the early pupal state in standard (25°C; 16 h light/8 h dark) and diapause-inducing (18°C; 8 h light/16 h dark) conditions. A forty-eight chip study (4 Expr12x135K slides) using cDNA from total RNA collected from two species in the Culex pipiens complex at 3 time-points during the early pupal stage to study gene expression differences between standard (25°C; 16 h light/8 h dark) and diapause-inducing (18°C; 8 h light/16 h dark) conditions, and between Culex pipiens (diapause) and C. quinquefasciatus (no diapause). Each chip measures the expression level of 18,692 protein coding genes, with 3 probes per gene and two-fold technical redundancy. Each of the 12-plex slides was used for one of four biological replicates. Probes were designed using the C.quinquefasciatus CpipJ1.2 geneset in VectorBase.
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