Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression data from CITED4 knockout and control mouse hearts after transverse aortic constriction

ABSTRACT: Gene expression data from CITED4 knockout and control mouse hearts after transverse aortic constriction

PROVIDER: PRJNA629314 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Gene expression data from CITED4 knockout and control mouse hearts after transverse aortic constriction

Project description:Rationale: Cardiac CITED4 is induced by exercise and is sufficient to cause physiological hypertrophy and mitigate adverse ventricular remodeling after ischemic injury. However, the role of endogenous CITED4 in response to physiological or pathological stress is unknown. Objective: To investigate the role of CITED4 in murine models of exercise and pressure overload. Methods and Results: We generated cardiomyocyte-specific CITED4 knockout mice (C4KO) and subjected them to an intensive swim exercise protocol as well as transverse aortic constriction (TAC). Echocardiography, western blotting, qPCR, immunohistochemistry, immunofluorescence, and transcriptional profiling for mRNA and miRNA expression were performed. Cellular crosstalk was investigated in vitro. CITED4 deletion in cardiomyocytes did not affect baseline cardiac size or function in young adult mice. C4KO mice developed modest cardiac dysfunction and dilation in response to exercise. After TAC, C4KOs developed severe heart failure with left ventricular dilation, impaired cardiomyocyte growth accompanied by reduced mammalian target of rapamycin (mTOR) activity and maladaptive cardiac remodeling with increased apoptosis, autophagy, and impaired mitochondrial signaling. Interstitial fibrosis was markedly increased in C4KO hearts after TAC. RNAseq revealed induction of a pro-fibrotic miRNA network. miR30d was decreased in C4KO hearts after TAC and mediated crosstalk between cardiomyocytes and fibroblasts to modulate fibrosis. miR30d inhibition was sufficient to increase cardiac dysfunction and fibrosis after TAC. Conclusions: CITED4 protects against pathological cardiac remodeling by regulating mTOR activity and a network of miRNAs mediating cardiomyocyte to fibroblast crosstalk. Our findings highlight the importance of CITED4 in response to both physiological and pathological stimuli.

2020-04-30 | GSE149584 | GEO

miRNA expression data from CITED4 knockout and control mouse hearts after transverse aortic constriction

Project description:miRNA expression data from CITED4 knockout and control mouse hearts after transverse aortic constriction

| PRJNA631727 | ENA

Proteome wide screening for ubiquitination, ISGylation and NEDDylation sites in wildtype and ISG15 knockout mouse hearts after transverse aortic constriction

Project description:Hearts(left ventricles) were harvested from control wildtype and ISG15 knockout mice and wildtype and ISG15 knockout mice 4 weeks after pressure overload induced by transverse aortic constriction. Nano LC-MS/MS analysis was performedon left ventricle tissue following protein extraction, trypsin digestion, peptide desalting, anti K-e-GG enrichment.

2023-05-10 | PXD032267 | Pride

Transcription profiling of hearts from FVB male mice subjected to cardiac pressure overload by transverse aortic constriction to study the quantitative relationships between gene expression and left ventricular hypertrophy

Project description:Expression profiling of hearts from FVB males subjected to cardiac pressure overload by transverse aortic constriction (TAC). TAC performed on 8-10 weeks month old males and females. Hearts examined 30 weeks after surgery.

2007-12-01 | E-GEOD-2459 | biostudies-arrayexpress

The effect of transverse aortic constriction on gene expression in Balb/cJ versus Balb/cByJ(∆ACADS) mice hearts

Project description:RNA-Seq data show that ACADS knockout curbs gene expression after transverse aortic constriction in mice

2021-05-11 | GSE165236 | GEO

mRNA Expression data from transverse aortic constriction hearts (cardiovascular disease) and sham hearts in mice.

2013-03-15 | GSE36407 | GEO

mRNA Expression data from transverse aortic constriction hearts (cardiovascular disease) and sham hearts in mice.

Project description:We used transverse aortic constraction pressure overload hypertrophy mouse hearts as a model of cardiovascular disease to study the genetic changes between TAC and SHAM (normal) mouse hearts and over 1 circadian cycle (24h). This is one approach to identify diurnal genetic biomarkers of cardiovascular disease. The micorarray approach allowed to see the gene expression in all genes in cardiovascular disease and sham hearts. There are 36 samples of cardiovascular disease (TAC) and normal SHAM hearts. For TAC: There were 3 mice sacrificed at each time point as biological replicates, for 6 timepoints over 24 hrs. For SHAM: There were 3 mice sacrificed at each time point as biological replicates, for 6 timepoints over 24 hrs.

2013-03-15 | E-GEOD-36407 | biostudies-arrayexpress

Metabolomic analysis in wildtype and Isg15 knockout mice after sham or transverse aortic constriction surgery

Project description:Left ventricular tissues were isolated from wildtype and Isg15 knockout mice 8 weeks after sham or transverse aortic constriction and the metabolomic differences were compared.

2023-01-07 | ST002437 | MetabolomicsWorkbench

RNA-seq data in cardiac tissues from cardiomyocyte-specific Acsl4 knockout mice after transverse aortic constriction

Project description:In order to investigate the differentially expressed genes in Acsl4 knockout after TAC (Transverse aortic constriction) or sham surgery. The experiment was divided to four groups including sham-operated Acsl4 flox/flox mice (FS), sham-operated Acsl4 knockout mice (KS), TAC-operated Acsl4 flox/flox mice (FT), TAC-operated Acsl4 knockout mice (KT) n=3 mice/group. The heart tissue was isolated after 21 days after performing TAC or sham surgery.

2024-08-26 | E-MTAB-14383 | biostudies-arrayexpress

Single-Cell Genomics of Transverse Aortic Constriction in Mouse

Project description:Single-Cell Genomics of Transverse Aortic Constriction in Mouse

| PRJNA529402 | ENA