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ABSTRACT: Cotton embryogenic callus

PROVIDER: PRJNA629328 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR11646203_1.fastq.gz	Fastqsanger.gz
	SRR11646203_2.fastq.gz	Fastqsanger.gz
	SRR11646204_1.fastq.gz	Fastqsanger.gz
	SRR11646204_2.fastq.gz	Fastqsanger.gz
	SRR11646205_1.fastq.gz	Fastqsanger.gz

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Differential small RNA expression upon induction and establishment of Tuxpeño maize embryogenic callus cultures

Project description:Purpose: Maize somatic embryogenesis is usually required to achieve genetic transformation and represents an important alternative in plant development. Although many embryogenesis-related genes have been studied in this model, the molecular mechanisms underlying cell dedifferentiation and further plant regeneration are not completely understood. Methods: Immature embryos smRNA profiles of 15-day-after-pollination (IE) and Embryogenic Callus from one (C1), four (C4), and ten months (C10) were generated by deep sequencing, using Illumina GAIIx. The sequence reads that passed quality filters were analyzed with two methods: Bowtie 1.1.2 and ShortStack 3.4. qRT–PCR validation for selected miRNAs was performed using SYBR Green assays. Results: We used high throughput sequencing to explore the sRNA populations during maize embryogenic callus induction and established subcultures from the Mexican cultivar VS-535, Tuxpeño landrace. We detected readjustments in 24 nt and 21-22 nt sRNA populations during the embryogenic callus establishment and maintenance. miRNAs related to stress response substantially increased upon callus proliferation establishment, correlating with a reduction in some of their target levels. On the other hand, while 24 nt-long hc-siRNAs derived from transposable retroelements transiently decreased in abundance during the embryogenic callus establishment, a population of 22 nt- hc-siRNAs increased. This was accompanied by reduction in transposon expression in the established callus subcultures. Conclusions: Stress- and development-related miRNAs are highly expressed upon maize EC callus induction and during maintenance subcultures, while miRNAs involved in hormone response only transiently increase during induction. The establishment of proliferative maize embryogenic callus is accompanied by important readjustments in the length of hc-siRNAs mapping to LTR retrotransposons, and their expression regulation.

2020-04-21 | GSE98055 | GEO

Embryogenic competence acquisition in sugarcane callus is associated with differential H+ pump abundance and activity

Project description:Somatic embryogenesis is an important biological process in several plant species, including sugarcane. Proteomics approaches have shown that H+ pumps are differentially regulated during somatic embryogenesis; however, the relationship between H+ flux and embryogenic competence is still unclear. This work aimed to elucidate the association between extracellular H+ flux and somatic embryo maturation in sugarcane. We performed a microsomal proteomics analysis and analyzed changes in extracellular H+ flux and H+ pump (P-H+-ATPase, V-H+-ATPase and H+-PPase) activity in embryogenic and non-embryogenic callus. A total of 657 proteins were identified, 16 of which were H+ pumps. We observed that P-H+-ATPase and H+-PPase were more abundant in embryogenic callus. Compared with non-embryogenic callus, embryogenic callus showed high H+ influx, especially at maturation day 14 as well as higher H+ pump activity, mainly P-H+-ATPase and H+-PPase activity. The H+-PPase appears to be the major H+ pump in embryogenic callus during somatic embryo formation, functioning in both vacuole acidification and PPi homeostasis. These results provide evidence for an association between higher H+ pump protein abundance and, consequently, higher H+ flux and embryogenic competence acquisition in the callus of sugarcane.

2018-06-26 | PXD009858 | Pride

embryogenesis col0-somatic embryogenesis

Project description:gnp07_regeneome_embryogenesis - embryogenesis col0 - Identify genes involved in somatic embryogenesis - compare embryogenic areas of a callus with undifferenciate area in the same callus

2012-06-01 | GSE30425 | GEO

embryogenesis ws-somatic embryogenesis

Project description:gnp07_regeneome_embryogenesis - embryogenesis ws - Identify genes involved in somatic embryogenesis - To compare embryogenic areas of a callus with undifferenciate area in the same callus

2012-06-01 | GSE30383 | GEO

embryogenesis col0-somatic embryogenesis

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embryogenesis ws-somatic embryogenesis

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embryogenesis ws-somatic embryogenesis

2013-04-11 | E-GEOD-45940 | biostudies-arrayexpress

Embryo development of Medicago truncatula

Project description:Early stage of embryo development from leaf-derived embryogenic callus of M. truncatula.

2002-11-19 | E-GEOD-74 | biostudies-arrayexpress

Embryo development of Medicago truncatula

Project description:Early stage of embryo development from leaf-derived embryogenic callus of M. truncatula. Keywords: parallel sample

2002-11-20 | GSE74 | GEO

small RNA-mediated regulation during Zea mays (maize) Somatic Embryogenesis [smRNA-Seq]

Project description:We report the role of sRNAs populations during the induction of callus tissues from VS-535 maize embryos displaying contrasting in vitro embryogenic potential; characterized through Next-generation sequencing (NGS). We conclude that the Embryogenic Response during Maize Somatic Embryogenesis induction is closely related to sRNAs regulation and depends on the developmental stage of the explant.

2019-10-21 | GSE120436 | GEO

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