Project description:Streptomyces bingchenggensis is a soil bacterium that produces milbemycins. Milbemycins and their derivatives are valuable biopesticides in the agricultural field. Owing to their advantages such as high efficiency and safety for human and animal,it was urgent to construct high-yield strain to ensure low production cost. To obtain genes closely correlated with milbemycin production, we have compared the whole genome microarray expression profiling of two strains (the parent one strain and high-yielding strain). In Streptomyces bingchenggensis, there are abundant exporters, which are responsible for transporting various substrates. In the result, some drug exporters were chosen to enhance production of milbemycin .
Project description:Streptomyces bingchenggensis is a soil bacterium that produces milbemycins. Milbemycins are commercially insecticidal and acaricidal antibiotics in agriculture, owing to their advantages such as high efficiency and safety for human and animal. To obtain genes valuable for further improvement of titer, we have compared two strains(the parental strain and high-yielding strain)by whole genome microarray expression profiling as a discovery platform. In Streptomyces bingchenggensis, there are abundant transporters, which are responsible for transporting various substrates. In the result, some sugar transporter genes showed significant gene expression.